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Int. J. Mol. Sci. 2014, 15(1), 1201-1215; doi:10.3390/ijms15011201
Article

Hispolon Decreases Melanin Production and Induces Apoptosis in Melanoma Cells through the Downregulation of Tyrosinase and Microphthalmia-Associated Transcription Factor (MITF) Expressions and the Activation of Caspase-3, -8 and -9

1,* , 2
,
1
 and
1
1 Department of Cosmetic Science, Providence University, 200 Chung-Chi Road, Shalu, Taichung 43301, Taiwan 2 Department of Cosmetic Science and Management, Mackay Medicine, Nursing and Management College, 92 Shengjing Road, Beitou, Taipei 11260, Taiwan
* Author to whom correspondence should be addressed.
Received: 27 November 2013 / Revised: 29 December 2013 / Accepted: 8 January 2014 / Published: 17 January 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Hispolon is one of the most important functional compounds that forms Phellinus linteus (Berkeley & Curtis) Teng. Hispolon has antioxidant, anti-inflammatory, antiproliferative and anticancer effects. In this study, we analyzed the functions of hispolon on melanogenesis and apoptosis in B16-F10 melanoma cells. The results demonstrated that hispolon is not an enzymatic inhibitor for tyrosinase; rather, it represses the expression of tyrosinase and the microphthalmia-associated transcription factor (MITF) to reduce the production of melanin in α-melanocyte-stimulating hormone (α-MSH)-stimulated B16-F10 cells at lower concentrations (less than 2 μM). In contrast, at higher concentration (greater than 10 μM), hispolon can induce activity of caspase-3, -8 and -9 to trigger apoptosis of B16-F10 cells but not of Detroit 551 normal fibroblast cells. Therefore, we suggest that hispolon has the potential to treat hyperpigmentation diseases and melanoma skin cancer in the future.
Keywords: apoptosis; caspase; hispolon; melanin; microphthalmia-associated transcription factor (MITF); tyrosinase apoptosis; caspase; hispolon; melanin; microphthalmia-associated transcription factor (MITF); tyrosinase
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Chen, Y.-S.; Lee, S.-M.; Lin, C.-C.; Liu, C.-Y. Hispolon Decreases Melanin Production and Induces Apoptosis in Melanoma Cells through the Downregulation of Tyrosinase and Microphthalmia-Associated Transcription Factor (MITF) Expressions and the Activation of Caspase-3, -8 and -9. Int. J. Mol. Sci. 2014, 15, 1201-1215.

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