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Int. J. Mol. Sci. 2014, 15(1), 1189-1200; doi:10.3390/ijms15011189
Communication

Clinical Evaluation of Extracellular ADMA Concentrations in Human Blood and Adipose Tissue

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Received: 10 December 2013; in revised form: 7 January 2014 / Accepted: 8 January 2014 / Published: 17 January 2014
(This article belongs to the Special Issue ADMA and Nitrergic System)
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Abstract: Circulating asymmetrical dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide synthesis, has been proposed as a biomarker for clinical outcome. Dimethylarginine dimethylaminohydrolase (DDAH) is the main enzyme responsible for ADMA metabolism and elimination. Adipose tissue ADMA concentrations and DDAH activity and their role in diabetes and obesity have not yet been investigated. In this study, we evaluated clinical microdialysis in combination with a sensitive analytical method (GC-MS/MS) to measure ADMA concentrations in extracellular fluid. Adipose tissue ADMA concentrations were assessed before and during an oral glucose tolerance test in lean healthy subjects and subjects with diabetes (n = 4 each), and in morbidly obese subjects before and after weight loss of 30 kg (n = 7). DDAH activity was determined in subcutaneous and visceral adipose tissue obtained during laparoscopic surgery (n = 5 paired samples). Mean interstitial ADMA concentrations did not differ between study populations (healthy 0.17 ± 0.03 µM; diabetic 0.21 ± 0.03 µM; morbidly obese 0.16 ± 0.01 and 0.17 ± 0.01 µM before and after weight loss, respectively). We did not observe any response of interstitial ADMA concentrations to the oral glucose challenge. Adipose tissue DDAH activity was negligible compared to liver tissue. Thus, adipose tissue ADMA plays a minor role in NO-dependent regulation of adipose tissue blood flow and metabolism.
Keywords: asymmetric dimethylarginine; nitric oxide; nitric oxide synthase; dimethylarginine dimethylaminohydrolase; adipose tissue; diabetes; obesity asymmetric dimethylarginine; nitric oxide; nitric oxide synthase; dimethylarginine dimethylaminohydrolase; adipose tissue; diabetes; obesity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

May, M.; Batkai, S.; Zörner, A.A.; Tsikas, D.; Jordan, J.; Engeli, S. Clinical Evaluation of Extracellular ADMA Concentrations in Human Blood and Adipose Tissue. Int. J. Mol. Sci. 2014, 15, 1189-1200.

AMA Style

May M, Batkai S, Zörner AA, Tsikas D, Jordan J, Engeli S. Clinical Evaluation of Extracellular ADMA Concentrations in Human Blood and Adipose Tissue. International Journal of Molecular Sciences. 2014; 15(1):1189-1200.

Chicago/Turabian Style

May, Marcus; Batkai, Sandor; Zörner, Alexander A.; Tsikas, Dimitrios; Jordan, Jens; Engeli, Stefan. 2014. "Clinical Evaluation of Extracellular ADMA Concentrations in Human Blood and Adipose Tissue." Int. J. Mol. Sci. 15, no. 1: 1189-1200.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert