Int. J. Mol. Sci. 2014, 15(1), 1080-1095; doi:10.3390/ijms15011080
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The Function and Catalysis of 2-Oxoglutarate-Dependent Oxygenases Involved in Plant Flavonoid Biosynthesis

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Received: 14 November 2013; in revised form: 26 December 2013 / Accepted: 29 December 2013 / Published: 15 January 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Flavonoids are secondary metabolites derived from phenylalanine and acetate metabolism. They fulfil a variety of functions in plants and have health benefits for humans. During the synthesis of the tricyclic flavonoid natural products in plants, oxidative modifications to the central C ring are catalyzed by four of FeII and 2-oxoglutarate dependent (2-ODD) oxygenases, namely flavone synthase I (FNS I), flavonol synthase (FLS), anthocyanidin synthase (ANS) and flavanone 3β-hydroxylase (FHT). FNS I, FLS and ANS are involved in desaturation of C2–C3 of flavonoids and FHT in hydroxylation of C3. FNS I, which is restricted to the Apiaceae species and in rice, is predicted to have evolved from FHT by duplication. Due to their sequence similarity and substrate specificity, FLS and ANS, which interact with the α surface of the substrate, belong to a group of dioxygenases having a broad substrate specificity, while FNS I and FHT are more selective, and interact with the naringenin β surface. Here, we summarize recent findings regarding the function of the four 2-ODD oxygenases and the relationship between their catalytic activity, their polypeptide sequence and their tertiary structure.
Keywords: flavonoid synthesis; 2-ODD oxygenases; flavone synthase I; flavonol synthase; anthocyanidin synthase; flavanone 3β-hydroxylase
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MDPI and ACS Style

Cheng, A.-X.; Han, X.-J.; Wu, Y.-F.; Lou, H.-X. The Function and Catalysis of 2-Oxoglutarate-Dependent Oxygenases Involved in Plant Flavonoid Biosynthesis. Int. J. Mol. Sci. 2014, 15, 1080-1095.

AMA Style

Cheng A-X, Han X-J, Wu Y-F, Lou H-X. The Function and Catalysis of 2-Oxoglutarate-Dependent Oxygenases Involved in Plant Flavonoid Biosynthesis. International Journal of Molecular Sciences. 2014; 15(1):1080-1095.

Chicago/Turabian Style

Cheng, Ai-Xia; Han, Xiao-Juan; Wu, Yi-Feng; Lou, Hong-Xiang. 2014. "The Function and Catalysis of 2-Oxoglutarate-Dependent Oxygenases Involved in Plant Flavonoid Biosynthesis." Int. J. Mol. Sci. 15, no. 1: 1080-1095.

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