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Int. J. Mol. Sci. 2014, 15(1), 1068-1079; doi:10.3390/ijms15011068

Identification of the High Molecular Weight Isoform of Phostensin

3,4,*  and 1,*
1 Department of Life Science and Institute of Molecular Biology, National Chung Cheng University, Chia-Yi 62102, Taiwan 2 Department of Nutrition and Health Science, Fooyin University, Kaohsiung 83102, Taiwan 3 Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei 11221, Taiwan 4 Department of Medical Research & Education, Taipei Veterans General Hospital, Taipei 11217, Taiwan These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 11 December 2013 / Revised: 31 December 2013 / Accepted: 6 January 2014 / Published: 15 January 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Phostensin is encoded by KIAA1949. 5'-RACEanalysis has been used to identify the translation start site of phostensin mRNA, indicating that it encodes 165 amino acids with an apparent molecular weight of 26 kDa on SDS-PAGE. This low-molecular-weight phostensin is present in human peripheral blood mononuclear cells and many leukemic cell lines. Phostensin is a protein phosphatase-1(PP1) binding protein. It also contains one actin-binding motif at its C-terminal region and binds to the pointed ends of actin filaments, modulating actin dynamics. In the current study, a high-molecular-weight phostensin is identified by using immunoprecipitationin combination with a proteomic approach. This new species of phostensin is also encoded by KIAA1949 and consists of 613 amino acids with an apparent molecular weight of 110 kDa on SDS-PAGE. The low-molecular-weight and high-molecular-weight phostensins were named as phostensin-α and phostensin-β, respectively. Although phostensin-α is the C-terminal region of phostensin-β, it is not degraded from phostensin-β. Phostensin-β is capable of associating with PP1 and actin filaments, and is present in many cell lines.
Keywords: phostensin-α; phostensin-β; KIAA1949; protein phosphatase-1; actin-binding protein phostensin-α; phostensin-β; KIAA1949; protein phosphatase-1; actin-binding protein
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Lin, Y.-S.; Huang, H.-L.; Liu, W.-T.; Lin, T.-H.; Huang, H.-B. Identification of the High Molecular Weight Isoform of Phostensin. Int. J. Mol. Sci. 2014, 15, 1068-1079.

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