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Identification of the High Molecular Weight Isoform of Phostensin
Department of Life Science and Institute of Molecular Biology, National Chung Cheng University, Chia-Yi 62102, Taiwan
Department of Nutrition and Health Science, Fooyin University, Kaohsiung 83102, Taiwan
Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei 11221, Taiwan
Department of Medical Research & Education, Taipei Veterans General Hospital, Taipei 11217, Taiwan
These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 11 December 2013; in revised form: 31 December 2013 / Accepted: 6 January 2014 / Published: 15 January 2014
Abstract: Phostensin is encoded by KIAA1949. 5'-RACEanalysis has been used to identify the translation start site of phostensin mRNA, indicating that it encodes 165 amino acids with an apparent molecular weight of 26 kDa on SDS-PAGE. This low-molecular-weight phostensin is present in human peripheral blood mononuclear cells and many leukemic cell lines. Phostensin is a protein phosphatase-1(PP1) binding protein. It also contains one actin-binding motif at its C-terminal region and binds to the pointed ends of actin filaments, modulating actin dynamics. In the current study, a high-molecular-weight phostensin is identified by using immunoprecipitationin combination with a proteomic approach. This new species of phostensin is also encoded by KIAA1949 and consists of 613 amino acids with an apparent molecular weight of 110 kDa on SDS-PAGE. The low-molecular-weight and high-molecular-weight phostensins were named as phostensin-α and phostensin-β, respectively. Although phostensin-α is the C-terminal region of phostensin-β, it is not degraded from phostensin-β. Phostensin-β is capable of associating with PP1 and actin filaments, and is present in many cell lines.
Keywords: phostensin-α; phostensin-β; KIAA1949; protein phosphatase-1; actin-binding protein
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Lin, Y.-S.; Huang, H.-L.; Liu, W.-T.; Lin, T.-H.; Huang, H.-B. Identification of the High Molecular Weight Isoform of Phostensin. Int. J. Mol. Sci. 2014, 15, 1068-1079.
Lin Y-S, Huang H-L, Liu W-T, Lin T-H, Huang H-B. Identification of the High Molecular Weight Isoform of Phostensin. International Journal of Molecular Sciences. 2014; 15(1):1068-1079.
Lin, Yu-Shan; Huang, Hsien-Lu; Liu, Wei-Ting; Lin, Ta-Hsien; Huang, Hsien-Bin. 2014. "Identification of the High Molecular Weight Isoform of Phostensin." Int. J. Mol. Sci. 15, no. 1: 1068-1079.