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Int. J. Mol. Sci. 2013, 14(9), 19230-19244; doi:10.3390/ijms140919230
Article

Analysis of Gln223Agr Polymorphism of Leptin Receptor Gene in Type II Diabetic Mellitus Subjects among Malaysians

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Received: 2 July 2013; in revised form: 26 August 2013 / Accepted: 27 August 2013 / Published: 18 September 2013
(This article belongs to the Section Molecular Pathology)
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Abstract: Leptin is known as the adipose peptide hormone. It plays an important role in the regulation of body fat and inhibits food intake by its action. Moreover, it is believed that leptin level deductions might be the cause of obesity and may play an important role in the development of Type 2 Diabetes Mellitus (T2DM), as well as in cardiovascular diseases (CVD). The Leptin Receptor (LEPR) gene and its polymorphisms have not been extensively studied in relation to the T2DM and its complications in various populations. In this study, we have determined the association of Gln223Agr loci of LEPR gene in three ethnic groups of Malaysia, namely: Malays, Chinese and Indians. A total of 284 T2DM subjects and 281 healthy individuals were recruited based on International Diabetes Federation (IDF) criteria. Genomic DNA was extracted from the buccal specimens of the subjects. The commercial polymerase chain reaction (PCR) method was carried out by proper restriction enzyme MSP I to both amplify and digest the Gln223Agr polymorphism. The p-value among the three studied races was 0.057, 0.011 and 0.095, respectively. The values such as age, WHR, FPG, HbA1C, LDL, HDL, Chol and Family History were significantly different among the subjects with Gln223Agr polymorphism of LEPR (p < 0.05).
Keywords: leptin receptor; polymorphism; type 2 diabetes mellitus; Malaysia; restriction enzyme leptin receptor; polymorphism; type 2 diabetes mellitus; Malaysia; restriction enzyme
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Etemad, A.; Ramachandran, V.; Pishva, S.R.; Heidari, F.; Aziz, A.F.A.; Yusof, A.K.M.; Pei, C.P.; Ismail, P. Analysis of Gln223Agr Polymorphism of Leptin Receptor Gene in Type II Diabetic Mellitus Subjects among Malaysians. Int. J. Mol. Sci. 2013, 14, 19230-19244.

AMA Style

Etemad A, Ramachandran V, Pishva SR, Heidari F, Aziz AFA, Yusof AKM, Pei CP, Ismail P. Analysis of Gln223Agr Polymorphism of Leptin Receptor Gene in Type II Diabetic Mellitus Subjects among Malaysians. International Journal of Molecular Sciences. 2013; 14(9):19230-19244.

Chicago/Turabian Style

Etemad, Ali; Ramachandran, Vasudevan; Pishva, Seyyed R.; Heidari, Farzad; Aziz, Ahmad F.A.; Yusof, Ahmad K.M.; Pei, Chong P.; Ismail, Patimah. 2013. "Analysis of Gln223Agr Polymorphism of Leptin Receptor Gene in Type II Diabetic Mellitus Subjects among Malaysians." Int. J. Mol. Sci. 14, no. 9: 19230-19244.


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