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Biohybrid Nanostructured Iron Oxide Nanoparticles and Satureja hortensis to Prevent Fungal Biofilm Development
Otorhinolaryngology, Carol Davila University of Medicine and Pharmacy, Traian Vuia no 6, Bucharest 020956, Romania
Department, Doctor Anghel Medical Center, Theodor Sperantia Street, Bucharest 30932, Romania
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, Bucharest 011061, Romania
Department of Microbiology and Immunology, Faculty of Biology, University of Bucharest, Bucharest 060101, Romania
* Author to whom correspondence should be addressed.
Received: 17 June 2013; in revised form: 5 August 2013 / Accepted: 23 August 2013 / Published: 4 September 2013
Abstract: Cutaneous wounds are often superinfected during the healing process and this leads to prolonged convalescence and discomfort. Usage of suitable wound dressings is very important for an appropriate wound care leading to a correct healing. The aim of this study was to demonstrate the influence of a nano-coated wound dressing (WD) on Candida albicans colonization rate and biofilm formation. The modified WD was achieved by submerging the dressing pieces into a nanofluid composed of functionalized magnetite nanoparticles and Satureja hortensis (SO) essential oil (EO). Chemical composition of the EO was established by GC-MS. The fabricated nanostructure was characterized by X-ray Diffraction (XRD), Transmission Electron Microscopy (TEM), Differential Thermal Analysis (DTA) and Fourier Transform-Infrared Spectroscopy (FT-IR). The analysis of the colonized surfaces using (Scanning Electron Microscopy) SEM revealed that C. albicans adherence and subsequent biofilm development are strongly inhibited on the surface of wound dressing fibers coated with the obtained nanofluid, comparing with regular uncoated materials. The results were also confirmed by the assay of the viable fungal cells embedded in the biofilm. Our data demonstrate that the obtained phytonanocoating improve the resistance of wound dressing surface to C. albicans colonization, which is often an etiological cause of local infections, impairing the appropriate wound healing.
Keywords: nano-modified wound dressing; Satureja hortensis essential oil; fungal biofilm; Candida albicans; magnetite nanoparticles; iron oxide
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MDPI and ACS Style
Anghel, I.; Grumezescu, A.M.; Holban, A.M.; Ficai, A.; Anghel, A.G.; Chifiriuc, M.C. Biohybrid Nanostructured Iron Oxide Nanoparticles and Satureja hortensis to Prevent Fungal Biofilm Development. Int. J. Mol. Sci. 2013, 14, 18110-18123.
Anghel I, Grumezescu AM, Holban AM, Ficai A, Anghel AG, Chifiriuc MC. Biohybrid Nanostructured Iron Oxide Nanoparticles and Satureja hortensis to Prevent Fungal Biofilm Development. International Journal of Molecular Sciences. 2013; 14(9):18110-18123.
Anghel, Ion; Grumezescu, Alexandru M.; Holban, Alina M.; Ficai, Anton; Anghel, Alina G.; Chifiriuc, Mariana C. 2013. "Biohybrid Nanostructured Iron Oxide Nanoparticles and Satureja hortensis to Prevent Fungal Biofilm Development." Int. J. Mol. Sci. 14, no. 9: 18110-18123.