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Int. J. Mol. Sci. 2013, 14(9), 17511-17524; doi:10.3390/ijms140917511
Article

Functional and Molecular Changes of the Bladder in Rats with Crushing Injury of Nerve Bundles from Major Pelvic Ganglion to the Bladder: Role of RhoA/Rho Kinase Pathway

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Received: 8 June 2013; in revised form: 20 July 2013 / Accepted: 5 August 2013 / Published: 27 August 2013
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Abstract: Voiding dysfunction is a common complication after radical pelvic surgery. To reduce this complication, nerve-sparing radical pelvic surgery was introduced. However, several patients experienced voiding difficulty despite nerve-sparing radical pelvic surgery. Thus, we investigated the functional and molecular changes of the bladder in rats, which demonstrated voiding dysfunction induced by nerve damage during nerve-sparing radical pelvic surgery. Male rats were used and assigned to normal, sham-operated, and bilateral crushing nerve bundles from major pelvic ganglion (MPG) to bladder group. After one, two, and four-week crushing injury, significantly decreased contractile response and increased connective tissue of the detrusor were observed and these results were reliable findings with voiding difficulty following nerve-sparing radical pelvic surgery. After crushing injury, significantly increased M2 muscarinic receptor expression was observed and this might be regarded as the compensatory response. However, M3 muscarinic receptor expression was not significantly changed. The expression of RhoA, ROCK-α, and ROCK-β was significantly increased after one, two, and four-week crushing injury. From these results, the down-regulation of RhoA/Rho kinase pathway might lead to the decreased bladder contractility after crushing injury of nerve bundles from MPG to the bladder despite of the compensated up-regulation of M2 muscarinic receptor.
Keywords: urinary bladder; receptor; muscarinic; Rho-associated kinase; autonomic ganglia; injury; nerve crush; rat urinary bladder; receptor; muscarinic; Rho-associated kinase; autonomic ganglia; injury; nerve crush; rat
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Kim, S.J.; Lee, D.S.; Bae, W.J.; Kim, S.; Hong, S.H.; Lee, J.Y.; Hwang, T.-K.; Kim, S.W. Functional and Molecular Changes of the Bladder in Rats with Crushing Injury of Nerve Bundles from Major Pelvic Ganglion to the Bladder: Role of RhoA/Rho Kinase Pathway. Int. J. Mol. Sci. 2013, 14, 17511-17524.

AMA Style

Kim SJ, Lee DS, Bae WJ, Kim S, Hong SH, Lee JY, Hwang T-K, Kim SW. Functional and Molecular Changes of the Bladder in Rats with Crushing Injury of Nerve Bundles from Major Pelvic Ganglion to the Bladder: Role of RhoA/Rho Kinase Pathway. International Journal of Molecular Sciences. 2013; 14(9):17511-17524.

Chicago/Turabian Style

Kim, Su J.; Lee, Dong S.; Bae, Woong J.; Kim, Seol; Hong, Sung H.; Lee, Ji Y.; Hwang, Tae-Kon; Kim, Sae W. 2013. "Functional and Molecular Changes of the Bladder in Rats with Crushing Injury of Nerve Bundles from Major Pelvic Ganglion to the Bladder: Role of RhoA/Rho Kinase Pathway." Int. J. Mol. Sci. 14, no. 9: 17511-17524.



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