Next Article in Journal
Assessment of Blood Flow in Hepatocellular Carcinoma: Correlations of Computed Tomography Perfusion Imaging and Circulating Angiogenic Factors
Next Article in Special Issue
The DNA-Damage Response to γ-Radiation Is Affected by miR-27a in A549 Cells
Previous Article in Journal
Functional and Molecular Changes of the Bladder in Rats with Crushing Injury of Nerve Bundles from Major Pelvic Ganglion to the Bladder: Role of RhoA/Rho Kinase Pathway
Previous Article in Special Issue
UVB-Stimulated TNFα Release from Human Melanocyte and Melanoma Cells Is Mediated by p38 MAPK
Int. J. Mol. Sci. 2013, 14(9), 17525-17535; doi:10.3390/ijms140917525

Cytogenetic Abnormalities in Lymphocytes from Victims Exposed to Cobalt-60 Radiation

, 4,†
, 1,2,3
, 1,2,3
, 1,2,3
, 1,2,3
, 5,6
, 5,6
, 1,2,3
, 1,2,3,*  and 1,2,3
Received: 24 June 2013 / Revised: 14 August 2013 / Accepted: 21 August 2013 / Published: 27 August 2013
(This article belongs to the collection Radiation Toxicity in Cells)
View Full-Text   |   Download PDF [266 KB, uploaded 19 June 2014]   |   Browse Figures


The present study investigates cytogenetic damage in lymphocytes, derived from three victims who were unfortunately exposed to cobalt-60 (60Co) radiation (the 1999 accident occurred in a village in China’s Henan province). Case A of the three victims was exposed to a higher dose of 60Co radiation than Cases B and C. The chromosomal aberrations, cytokinesis-block micronucleus (CBMN, the CBMN assay), and DNA double-strand breaks (DSBs, the comet assay) examined in this study are biomarkers for cytogenetic abnormalities. After the lymphocytes collected from the victims were cultured, the frequencies of dicentric chromosomes and rings (dic + r) and CBMN in the first mitotic division detected in the lymphocytes of Case A were found to be substantially higher than in Cases B and C. Similarly, the DNA-DSB level found in the peripheral blood collected from Case A was much higher than those of Cases B and C. These results suggest that an acutely enhanced induction of the 60Co-induced cytogenetic abnormality frequency in humans depends on the dose of 60Co radiation. This finding is supported by the data obtained using practical techniques to evaluate early lymphoid-tissue abnormalities induced after exposure to acute radiation.
Keywords: radiation accident; chromosome aberration; micronucleus assay; comet assay; DNA-DSB radiation accident; chromosome aberration; micronucleus assay; comet assay; DNA-DSB
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
MDPI and ACS Style

Cao, J.; Zhang, J.; Wang, Y.; Du, L.Q.; Xu, C.; Wang, Q.; Liu, J.X.; Su, X.; Fan, F.Y.; Liu, Q.; Fan, S.J. Cytogenetic Abnormalities in Lymphocytes from Victims Exposed to Cobalt-60 Radiation. Int. J. Mol. Sci. 2013, 14, 17525-17535.

View more citation formats

Related Articles

Article Metrics


Citing Articles

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert