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Cytogenetic Abnormalities in Lymphocytes from Victims Exposed to Cobalt-60 Radiation
Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China
Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China
First Clinical Department of Medical Emergency Response Centre for Nuclear Accident, Ministry of Health, Tianjin 300192, China
Tianjin Third Central Hospital, Tianjin 300170, China
National Institute for Radiological Protection and Nuclear Safety, Chinese Centre for Disease Control, Beijing 100088, China
Medical Emergency Response Centre for Nuclear Accident, Ministry of Health, Beijing 100088, China
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 24 June 2013; in revised form: 14 August 2013 / Accepted: 21 August 2013 / Published: 27 August 2013
Abstract: The present study investigates cytogenetic damage in lymphocytes, derived from three victims who were unfortunately exposed to cobalt-60 (60Co) radiation (the 1999 accident occurred in a village in China’s Henan province). Case A of the three victims was exposed to a higher dose of 60Co radiation than Cases B and C. The chromosomal aberrations, cytokinesis-block micronucleus (CBMN, the CBMN assay), and DNA double-strand breaks (DSBs, the comet assay) examined in this study are biomarkers for cytogenetic abnormalities. After the lymphocytes collected from the victims were cultured, the frequencies of dicentric chromosomes and rings (dic + r) and CBMN in the first mitotic division detected in the lymphocytes of Case A were found to be substantially higher than in Cases B and C. Similarly, the DNA-DSB level found in the peripheral blood collected from Case A was much higher than those of Cases B and C. These results suggest that an acutely enhanced induction of the 60Co-induced cytogenetic abnormality frequency in humans depends on the dose of 60Co radiation. This finding is supported by the data obtained using practical techniques to evaluate early lymphoid-tissue abnormalities induced after exposure to acute radiation.
Keywords: radiation accident; chromosome aberration; micronucleus assay; comet assay; DNA-DSB
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Cao, J.; Zhang, J.; Wang, Y.; Du, L.Q.; Xu, C.; Wang, Q.; Liu, J.X.; Su, X.; Fan, F.Y.; Liu, Q.; Fan, S.J. Cytogenetic Abnormalities in Lymphocytes from Victims Exposed to Cobalt-60 Radiation. Int. J. Mol. Sci. 2013, 14, 17525-17535.
Cao J, Zhang J, Wang Y, Du LQ, Xu C, Wang Q, Liu JX, Su X, Fan FY, Liu Q, Fan SJ. Cytogenetic Abnormalities in Lymphocytes from Victims Exposed to Cobalt-60 Radiation. International Journal of Molecular Sciences. 2013; 14(9):17525-17535.
Cao, Jia; Zhang, Jing; Wang, Yan; Du, Li Q.; Xu, Chang; Wang, Qin; Liu, Jian X.; Su, Xu; Fan, Fei Y.; Liu, Qiang; Fan, Sai J. 2013. "Cytogenetic Abnormalities in Lymphocytes from Victims Exposed to Cobalt-60 Radiation." Int. J. Mol. Sci. 14, no. 9: 17525-17535.