Int. J. Mol. Sci. 2013, 14(9), 17525-17535; doi:10.3390/ijms140917525

Cytogenetic Abnormalities in Lymphocytes from Victims Exposed to Cobalt-60 Radiation

1,2,3,†email, 4,†email, 1,2,3email, 1,2,3email, 1,2,3email, 1,2,3email, 5,6email, 5,6email, 1,2,3email, 1,2,3,* email and 1,2,3email
Received: 24 June 2013; in revised form: 14 August 2013 / Accepted: 21 August 2013 / Published: 27 August 2013
(This article belongs to the collection Radiation Toxicity in Cells)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The present study investigates cytogenetic damage in lymphocytes, derived from three victims who were unfortunately exposed to cobalt-60 (60Co) radiation (the 1999 accident occurred in a village in China’s Henan province). Case A of the three victims was exposed to a higher dose of 60Co radiation than Cases B and C. The chromosomal aberrations, cytokinesis-block micronucleus (CBMN, the CBMN assay), and DNA double-strand breaks (DSBs, the comet assay) examined in this study are biomarkers for cytogenetic abnormalities. After the lymphocytes collected from the victims were cultured, the frequencies of dicentric chromosomes and rings (dic + r) and CBMN in the first mitotic division detected in the lymphocytes of Case A were found to be substantially higher than in Cases B and C. Similarly, the DNA-DSB level found in the peripheral blood collected from Case A was much higher than those of Cases B and C. These results suggest that an acutely enhanced induction of the 60Co-induced cytogenetic abnormality frequency in humans depends on the dose of 60Co radiation. This finding is supported by the data obtained using practical techniques to evaluate early lymphoid-tissue abnormalities induced after exposure to acute radiation.
Keywords: radiation accident; chromosome aberration; micronucleus assay; comet assay; DNA-DSB
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MDPI and ACS Style

Cao, J.; Zhang, J.; Wang, Y.; Du, L.Q.; Xu, C.; Wang, Q.; Liu, J.X.; Su, X.; Fan, F.Y.; Liu, Q.; Fan, S.J. Cytogenetic Abnormalities in Lymphocytes from Victims Exposed to Cobalt-60 Radiation. Int. J. Mol. Sci. 2013, 14, 17525-17535.

AMA Style

Cao J, Zhang J, Wang Y, Du LQ, Xu C, Wang Q, Liu JX, Su X, Fan FY, Liu Q, Fan SJ. Cytogenetic Abnormalities in Lymphocytes from Victims Exposed to Cobalt-60 Radiation. International Journal of Molecular Sciences. 2013; 14(9):17525-17535.

Chicago/Turabian Style

Cao, Jia; Zhang, Jing; Wang, Yan; Du, Li Q.; Xu, Chang; Wang, Qin; Liu, Jian X.; Su, Xu; Fan, Fei Y.; Liu, Qiang; Fan, Sai J. 2013. "Cytogenetic Abnormalities in Lymphocytes from Victims Exposed to Cobalt-60 Radiation." Int. J. Mol. Sci. 14, no. 9: 17525-17535.

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