Int. J. Mol. Sci. 2013, 14(7), 14689-14699; doi:10.3390/ijms140714689
Article

Cascading cis-Cleavage on Transcript from trans-Acting siRNA-Producing Locus 3

1,†,* email, 2,†email, 3email, 1email and 1email
Received: 2 May 2013; in revised form: 24 June 2013 / Accepted: 4 July 2013 / Published: 12 July 2013
(This article belongs to the Special Issue Regulation by non-coding RNAs)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The production of small RNAs (sRNAs) from phased positions set by microRNA-directed cleavage of trans-acting-siRNA-producing locus (TAS) transcript has been characterized extensively; however, the production of sRNAs from non-phased positions remains unknown. We report three cis-cleavages that occurred in TAS3 transcripts in Vitis vinifera, by combining high-throughput sRNA deep sequencing information with evolutional conservation and genome-wide RNA degradome analysis. The three cis-cleavages can be deciphered to generate an orderly cleavage cascade, and can also produce distinct phasing patterns. Each of the patterns, either upstream or downstream of the cis-cleaved position, had a set of sRNAs arranged in 21-nucleotide increments. Part of the cascading cis-cleavages was also conserved in Arabidopsis thaliana. Our results will enhance the understanding of the production of sRNAs from non-phased positions that are not set by microRNA-directed cleavage.
Keywords: trans-acting siRNA; cis-cleavage; ta-siRNA-producing locus; miRNA
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MDPI and ACS Style

Zhang, C.; Li, G.; Wang, J.; Zhu, S.; Li, H. Cascading cis-Cleavage on Transcript from trans-Acting siRNA-Producing Locus 3. Int. J. Mol. Sci. 2013, 14, 14689-14699.

AMA Style

Zhang C, Li G, Wang J, Zhu S, Li H. Cascading cis-Cleavage on Transcript from trans-Acting siRNA-Producing Locus 3. International Journal of Molecular Sciences. 2013; 14(7):14689-14699.

Chicago/Turabian Style

Zhang, Changqing; Li, Guangping; Wang, Jin; Zhu, Shinong; Li, Hailing. 2013. "Cascading cis-Cleavage on Transcript from trans-Acting siRNA-Producing Locus 3." Int. J. Mol. Sci. 14, no. 7: 14689-14699.

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