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Int. J. Mol. Sci. 2013, 14(7), 14076-14084; doi:10.3390/ijms140714076

MicroRNA-27a Is Induced by Leucine and Contributes to Leucine-Induced Proliferation Promotion in C2C12 Cells

* ,
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
* Author to whom correspondence should be addressed.
Received: 17 May 2013 / Revised: 30 May 2013 / Accepted: 26 June 2013 / Published: 8 July 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Leucine, a branched chain amino acid, is well known to stimulate protein synthesis in skeletal muscle. However, the role of leucine in myoblast proliferation remains unclear. In this study, we found that leucine could promote proliferation of C2C12 cells. Moreover, expressions of miR-27a and myostatin (a bona fide target of miR-27a) were upregulated and downregulated, respectively, following leucine treatment. We also found that miR-27a loss-of-function by transfection of a miR-27a inhibitor suppressed the promotion of myoblast proliferation caused by leucine. Our results suggest that miR-27a is induced by leucine and contributes to leucine-induced proliferation promotion of myoblast.
Keywords: leucine; C2C12 cells; miR-27a; myoblast proliferation leucine; C2C12 cells; miR-27a; myoblast proliferation
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Chen, X.; Huang, Z.; Chen, D.; Yang, T.; Liu, G. MicroRNA-27a Is Induced by Leucine and Contributes to Leucine-Induced Proliferation Promotion in C2C12 Cells. Int. J. Mol. Sci. 2013, 14, 14076-14084.

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