Next Article in Journal
Presence of proNGF-Sortilin Signaling Complex in Nigral Dopamine Neurons and Its Variation in Relation to Aging, Lactacystin and 6-OHDA Insults
Previous Article in Journal
Effects of Ospemifene on Drug Metabolism Mediated by Cytochrome P450 Enzymes in Humans in Vitro and in Vivo
Int. J. Mol. Sci. 2013, 14(7), 14076-14084; doi:10.3390/ijms140714076
Article

MicroRNA-27a Is Induced by Leucine and Contributes to Leucine-Induced Proliferation Promotion in C2C12 Cells

, * ,
,
 and
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
* Author to whom correspondence should be addressed.
Received: 17 May 2013 / Revised: 30 May 2013 / Accepted: 26 June 2013 / Published: 8 July 2013
(This article belongs to the Section Bioinorganic Chemistry)
View Full-Text   |   Download PDF [614 KB, uploaded 19 June 2014]   |   Browse Figures

Abstract

Leucine, a branched chain amino acid, is well known to stimulate protein synthesis in skeletal muscle. However, the role of leucine in myoblast proliferation remains unclear. In this study, we found that leucine could promote proliferation of C2C12 cells. Moreover, expressions of miR-27a and myostatin (a bona fide target of miR-27a) were upregulated and downregulated, respectively, following leucine treatment. We also found that miR-27a loss-of-function by transfection of a miR-27a inhibitor suppressed the promotion of myoblast proliferation caused by leucine. Our results suggest that miR-27a is induced by leucine and contributes to leucine-induced proliferation promotion of myoblast.
Keywords: leucine; C2C12 cells; miR-27a; myoblast proliferation leucine; C2C12 cells; miR-27a; myoblast proliferation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Export to BibTeX |
EndNote


MDPI and ACS Style

Chen, X.; Huang, Z.; Chen, D.; Yang, T.; Liu, G. MicroRNA-27a Is Induced by Leucine and Contributes to Leucine-Induced Proliferation Promotion in C2C12 Cells. Int. J. Mol. Sci. 2013, 14, 14076-14084.

View more citation formats

Related Articles

Article Metrics

Comments

Citing Articles

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert