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Int. J. Mol. Sci. 2013, 14(7), 13377-13390; doi:10.3390/ijms140713377
Article

A Labile Pool of IQGAP1 Disassembles Endothelial Adherens Junctions

1,†
, 2,3,†
 and 2,*
1 Department of Anesthesiology, 1st Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning, China 2 School of Bioscience & Bioengineering, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, Guangdong, China 3 Nanotides Inc., 401 Professional Drive, Suite 130, Gaithersburg, MD 20879, USA These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 10 April 2013 / Revised: 20 June 2013 / Accepted: 21 June 2013 / Published: 27 June 2013
(This article belongs to the Section Bioinorganic Chemistry)
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Abstract

Adhesion molecules are known to play an important role in endothelial activation and angiogenesis. Here we determined the functional role of IQGAP1 in the regulation of endothelial adherens junctions. VE-cadherin is found to be associated with actin filaments and thus stable, but IQGAP1 at intercellular junctions is not bound to actin filaments and thus labile. Expression of GFP labeled VE-α-catenin is shown to increase the electrical resistance across HUVEC monolayers and diminishes endogenous labile IQGAP1 at the intercellular junctions. Knockdown of endogenous IQGAP1 enhances intercellular adhesion in HUVECs by increasing the association of VE-cadherin with P120 and β-catenin. IQGAP1 knockdown also decreases the interaction of N-cadherin with P120 and β-catenin. Together, these results suggest that a labile pool of IQGAP1 at intercellular junctions disassembles adherens junctions and thus impairs endothelial cell-cell adhesion.
Keywords: IQGAP1; Endothelial Adherens Junction; VE-cadherin; N-cadherin IQGAP1; Endothelial Adherens Junction; VE-cadherin; N-cadherin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Yuan, Z.; Zhang, W.; Tan, W. A Labile Pool of IQGAP1 Disassembles Endothelial Adherens Junctions. Int. J. Mol. Sci. 2013, 14, 13377-13390.

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