Next Article in Journal
Comparison of Membrane Targeting Strategies for the Accumulation of the Human Immunodeficiency Virus p24 Protein in Transgenic Tobacco
Next Article in Special Issue
The Intertwining of Transposable Elements and Non-Coding RNAs
Previous Article in Journal
Toll-Like Receptor and Accessory Molecule mRNA Expression in Humans and Mice as Well as in Murine Autoimmunity, Transient Inflammation, and Progressive Fibrosis
Previous Article in Special Issue
MicroRNA Regulation in Renal Pathophysiology
Int. J. Mol. Sci. 2013, 14(7), 13231-13240; doi:10.3390/ijms140713231

Up-Regulation of microRNA* Strands by Their Target Transcripts

Received: 27 April 2013 / Revised: 29 May 2013 / Accepted: 17 June 2013 / Published: 26 June 2013
(This article belongs to the Special Issue Regulation by non-coding RNAs 2013)
View Full-Text   |   Download PDF [829 KB, uploaded 19 June 2014]   |   Browse Figures


During microRNA (miRNA) biogenesis, one strand of a 21–23 nucleotide RNA duplex is preferentially selected for entry into an RNA-induced silencing complex (RISC). The other strand, known as the miRNA* species, is typically thought to be degraded. Previous studies have provided miRNA* selection models, but it remains unclear how the dominance of one arm arises during the biogenesis of miRNA. Using miRNA sponge-like methods, we cloned four tandem target sequences (artificial target) of miR-7b* and then measured miR-7b* expression levels after transfection of the artificial target. miR-7b* levels were found to significantly increase after transfection of the artificial target. We postulate that the abundance of target transcripts drives miRNA arm selection.
Keywords: microRNA (miRNA); pre-miRNA; RISC; Drosha; Dicer; Argonaute (Ago) microRNA (miRNA); pre-miRNA; RISC; Drosha; Dicer; Argonaute (Ago)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
MDPI and ACS Style

Kang, S.-M.; Choi, J.-W.; Hong, S.-H.; Lee, H.-J. Up-Regulation of microRNA* Strands by Their Target Transcripts. Int. J. Mol. Sci. 2013, 14, 13231-13240.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


Cited By

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert