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Up-Regulation of microRNA* Strands by Their Target Transcripts
Department of Oral Microbiology, School of Dentistry, Kyungpook National University, Daegu 700-412, Korea
Brain Science and Engineering Institute, Kyungpook National University, Daegu 700-412, Korea
* Author to whom correspondence should be addressed.
Received: 27 April 2013; in revised form: 29 May 2013 / Accepted: 17 June 2013 / Published: 26 June 2013
Abstract: During microRNA (miRNA) biogenesis, one strand of a 21–23 nucleotide RNA duplex is preferentially selected for entry into an RNA-induced silencing complex (RISC). The other strand, known as the miRNA* species, is typically thought to be degraded. Previous studies have provided miRNA* selection models, but it remains unclear how the dominance of one arm arises during the biogenesis of miRNA. Using miRNA sponge-like methods, we cloned four tandem target sequences (artificial target) of miR-7b* and then measured miR-7b* expression levels after transfection of the artificial target. miR-7b* levels were found to significantly increase after transfection of the artificial target. We postulate that the abundance of target transcripts drives miRNA arm selection.
Keywords: microRNA (miRNA); pre-miRNA; RISC; Drosha; Dicer; Argonaute (Ago)
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Kang, S.-M.; Choi, J.-W.; Hong, S.-H.; Lee, H.-J. Up-Regulation of microRNA* Strands by Their Target Transcripts. Int. J. Mol. Sci. 2013, 14, 13231-13240.
Kang S-M, Choi J-W, Hong S-H, Lee H-J. Up-Regulation of microRNA* Strands by Their Target Transcripts. International Journal of Molecular Sciences. 2013; 14(7):13231-13240.
Kang, Sung-Min; Choi, Ji-Woong; Hong, Su-Hyung; Lee, Heon-Jin. 2013. "Up-Regulation of microRNA* Strands by Their Target Transcripts." Int. J. Mol. Sci. 14, no. 7: 13231-13240.