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Int. J. Mol. Sci. 2013, 14(7), 13231-13240; doi:10.3390/ijms140713231

Up-Regulation of microRNA* Strands by Their Target Transcripts

1
Department of Oral Microbiology, School of Dentistry, Kyungpook National University, Daegu 700-412, Korea
2
Brain Science and Engineering Institute, Kyungpook National University, Daegu 700-412, Korea
*
Author to whom correspondence should be addressed.
Received: 27 April 2013 / Revised: 29 May 2013 / Accepted: 17 June 2013 / Published: 26 June 2013
(This article belongs to the Special Issue Regulation by non-coding RNAs 2013)
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Abstract

During microRNA (miRNA) biogenesis, one strand of a 21–23 nucleotide RNA duplex is preferentially selected for entry into an RNA-induced silencing complex (RISC). The other strand, known as the miRNA* species, is typically thought to be degraded. Previous studies have provided miRNA* selection models, but it remains unclear how the dominance of one arm arises during the biogenesis of miRNA. Using miRNA sponge-like methods, we cloned four tandem target sequences (artificial target) of miR-7b* and then measured miR-7b* expression levels after transfection of the artificial target. miR-7b* levels were found to significantly increase after transfection of the artificial target. We postulate that the abundance of target transcripts drives miRNA arm selection.
Keywords: microRNA (miRNA); pre-miRNA; RISC; Drosha; Dicer; Argonaute (Ago) microRNA (miRNA); pre-miRNA; RISC; Drosha; Dicer; Argonaute (Ago)
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Kang, S.-M.; Choi, J.-W.; Hong, S.-H.; Lee, H.-J. Up-Regulation of microRNA* Strands by Their Target Transcripts. Int. J. Mol. Sci. 2013, 14, 13231-13240.

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