Int. J. Mol. Sci. 2013, 14(7), 13231-13240; doi:10.3390/ijms140713231
Communication

Up-Regulation of microRNA* Strands by Their Target Transcripts

1email, 1email, 1email and 1,2,* email
Received: 27 April 2013; in revised form: 29 May 2013 / Accepted: 17 June 2013 / Published: 26 June 2013
(This article belongs to the Special Issue Regulation by non-coding RNAs)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: During microRNA (miRNA) biogenesis, one strand of a 21–23 nucleotide RNA duplex is preferentially selected for entry into an RNA-induced silencing complex (RISC). The other strand, known as the miRNA* species, is typically thought to be degraded. Previous studies have provided miRNA* selection models, but it remains unclear how the dominance of one arm arises during the biogenesis of miRNA. Using miRNA sponge-like methods, we cloned four tandem target sequences (artificial target) of miR-7b* and then measured miR-7b* expression levels after transfection of the artificial target. miR-7b* levels were found to significantly increase after transfection of the artificial target. We postulate that the abundance of target transcripts drives miRNA arm selection.
Keywords: microRNA (miRNA); pre-miRNA; RISC; Drosha; Dicer; Argonaute (Ago)
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MDPI and ACS Style

Kang, S.-M.; Choi, J.-W.; Hong, S.-H.; Lee, H.-J. Up-Regulation of microRNA* Strands by Their Target Transcripts. Int. J. Mol. Sci. 2013, 14, 13231-13240.

AMA Style

Kang S-M, Choi J-W, Hong S-H, Lee H-J. Up-Regulation of microRNA* Strands by Their Target Transcripts. International Journal of Molecular Sciences. 2013; 14(7):13231-13240.

Chicago/Turabian Style

Kang, Sung-Min; Choi, Ji-Woong; Hong, Su-Hyung; Lee, Heon-Jin. 2013. "Up-Regulation of microRNA* Strands by Their Target Transcripts." Int. J. Mol. Sci. 14, no. 7: 13231-13240.

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