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Cloning and Polymorphisms of Yak Lactate Dehydrogenase b Gene
College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China
College of Animal Science, China Agricultural University, Beijing 100193, China
College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA
* Author to whom correspondence should be addressed.
Received: 4 March 2013; in revised form: 21 May 2013 / Accepted: 29 May 2013 / Published: 5 June 2013
Abstract: The main objective of this work was to study the unique polymorphisms of the lactate dehydrogenase-1 (LDH1) gene in yak (Bos grunniens). Native polyacrylamide gel electrophoresis revealed three phenotypes of LDH1 (a tetramer of H subunit) in yak heart and longissimus muscle extracts. The corresponding gene, ldhb, encoding H subunits of three LDH1 phenotypes was obtained by RT-PCR. A total of six nucleotide differences were detected in yak ldhb compared with that of cattle, of which five mutations cause amino acid substitutions. Sequence analysis shows that the G896A and C689A, mutations of ldhb gene, result in alterations of differently charged amino acids, and create the three phenotypes (F, M, and S) of yak LDH1. Molecular modeling of the H subunit of LDH indicates that the substituted amino acids are not located within NAD+ or substrate binding sites. PCR-RFLP examination of G896A mutation demonstrated that most LDH1-F samples are actually heterozygote at this site. These results help to elucidate the molecular basis and genetic characteristic of the three unique LDH1 phenotypes in yak.
Keywords: Bos grunniens; lactate dehydrogenase; polymorphism; hypoxic adaptation
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MDPI and ACS Style
Wang, G.; Zhao, X.; Zhong, J.; Cao, M.; He, Q.; Liu, Z.; Lin, Y.; Xu, Y.; Zheng, Y. Cloning and Polymorphisms of Yak Lactate Dehydrogenase b Gene. Int. J. Mol. Sci. 2013, 14, 11994-12003.
Wang G, Zhao X, Zhong J, Cao M, He Q, Liu Z, Lin Y, Xu Y, Zheng Y. Cloning and Polymorphisms of Yak Lactate Dehydrogenase b Gene. International Journal of Molecular Sciences. 2013; 14(6):11994-12003.
Wang, Guosheng; Zhao, Xingbo; Zhong, Juming; Cao, Meng; He, Qinghua; Liu, Zhengxin; Lin, Yaqiu; Xu, Yaou; Zheng, Yucai. 2013. "Cloning and Polymorphisms of Yak Lactate Dehydrogenase b Gene." Int. J. Mol. Sci. 14, no. 6: 11994-12003.