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Special Issue "Advances in Cellular and Molecular Life Sciences"

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A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Biochemistry, Molecular Biology and Biophysics".

Deadline for manuscript submissions: closed (31 March 2011)

Special Issue Editor

Guest Editor
Prof. Dr. Geoffrey Burnstock (Website)

Autonomic Neuroscience Centre, Royal Free and University College Medical School, Rowland Hill Street, London NW3 2PF, UK

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Research

Open AccessArticle Cloning and Polymorphisms of Yak Lactate Dehydrogenase b Gene
Int. J. Mol. Sci. 2013, 14(6), 11994-12003; doi:10.3390/ijms140611994
Received: 4 March 2013 / Revised: 21 May 2013 / Accepted: 29 May 2013 / Published: 5 June 2013
Cited by 1 | PDF Full-text (621 KB) | HTML Full-text | XML Full-text
Abstract
The main objective of this work was to study the unique polymorphisms of the lactate dehydrogenase-1 (LDH1) gene in yak (Bos grunniens). Native polyacrylamide gel electrophoresis revealed three phenotypes of LDH1 (a tetramer of H subunit) in yak heart and [...] Read more.
The main objective of this work was to study the unique polymorphisms of the lactate dehydrogenase-1 (LDH1) gene in yak (Bos grunniens). Native polyacrylamide gel electrophoresis revealed three phenotypes of LDH1 (a tetramer of H subunit) in yak heart and longissimus muscle extracts. The corresponding gene, ldhb, encoding H subunits of three LDH1 phenotypes was obtained by RT-PCR. A total of six nucleotide differences were detected in yak ldhb compared with that of cattle, of which five mutations cause amino acid substitutions. Sequence analysis shows that the G896A and C689A, mutations of ldhb gene, result in alterations of differently charged amino acids, and create the three phenotypes (F, M, and S) of yak LDH1. Molecular modeling of the H subunit of LDH indicates that the substituted amino acids are not located within NAD+ or substrate binding sites. PCR-RFLP examination of G896A mutation demonstrated that most LDH1-F samples are actually heterozygote at this site. These results help to elucidate the molecular basis and genetic characteristic of the three unique LDH1 phenotypes in yak. Full article
(This article belongs to the Special Issue Advances in Cellular and Molecular Life Sciences)

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