Int. J. Mol. Sci. 2013, 14(6), 10852-10868; doi:10.3390/ijms140610852
Article

Generation of Magnetized Olfactory Ensheathing Cells for Regenerative Studies in the Central and Peripheral Nervous Tissue

1,†,* email, 2,3,4,†email, 5email, 5email, 1email, 1,6email and 2,3,4email
Received: 1 April 2013; in revised form: 8 May 2013 / Accepted: 13 May 2013 / Published: 24 May 2013
(This article belongs to the Special Issue Magnetic Nanoparticles 2013)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: As olfactory receptor axons grow from the peripheral to the central nervous system (CNS) aided by olfactory ensheathing cells (OECs), the transplantation of OECs has been suggested as a plausible therapy for spinal cord lesions. The problem with this hypothesis is that OECs do not represent a single homogeneous entity, but, instead, a functionally heterogeneous population that exhibits a variety of responses, including adhesion and repulsion during cell-matrix interactions. Some studies report that the migratory properties of OECs are compromised by inhibitory molecules and potentiated by chemical gradients. In this paper, we report a system based on modified OECs carrying magnetic nanoparticles as a proof of concept experiment enabling specific studies aimed at exploring the potential of OECs in the treatment of spinal cord injuries. Our studies have confirmed that magnetized OECs (i) survive well without exhibiting stress-associated cellular responses; (ii) in vitro, their migration can be modulated by magnetic fields; and (iii) their transplantation in organotypic slices of spinal cord and peripheral nerve showed positive integration in the model. Altogether, these findings indicate the therapeutic potential of magnetized OECs for CNS injuries.
Keywords: nerve regeneration; olfactory ensheathing cell; magnetic nanoparticle; organotypic culture
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MDPI and ACS Style

Riggio, C.; Nocentini, S.; Catalayud, M.P.; Goya, G.F.; Cuschieri, A.; Raffa, V.; del Río, J.A. Generation of Magnetized Olfactory Ensheathing Cells for Regenerative Studies in the Central and Peripheral Nervous Tissue. Int. J. Mol. Sci. 2013, 14, 10852-10868.

AMA Style

Riggio C, Nocentini S, Catalayud MP, Goya GF, Cuschieri A, Raffa V, del Río JA. Generation of Magnetized Olfactory Ensheathing Cells for Regenerative Studies in the Central and Peripheral Nervous Tissue. International Journal of Molecular Sciences. 2013; 14(6):10852-10868.

Chicago/Turabian Style

Riggio, Cristina; Nocentini, Sara; Catalayud, Maria P.; Goya, Gerardo F.; Cuschieri, Alfred; Raffa, Vittoria; del Río, José A. 2013. "Generation of Magnetized Olfactory Ensheathing Cells for Regenerative Studies in the Central and Peripheral Nervous Tissue." Int. J. Mol. Sci. 14, no. 6: 10852-10868.


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