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The Effect of the Aerial Part of Lindera akoensis on Lipopolysaccharides (LPS)-Induced Nitric Oxide Production in RAW264.7 Cells
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan
Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung 912, Taiwan
Department of Forestry, National Chung Hsing University, Taichung 402, Taiwan
Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan
Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan
The Experimental Forest Management Office, National Chung-Hsing University, Taichung 402, Taiwan
Tsuzuki Institute for Traditional Medicine, China Medical University, Taichung 402, Taiwan
* Authors to whom correspondence should be addressed.
Received: 12 March 2013; in revised form: 12 April 2013 / Accepted: 15 April 2013 / Published: 26 April 2013
Abstract: Four new secondary metabolites, 3α-((E)-Dodec-1-enyl)-4β-hydroxy-5β-methyldihydrofuran-2-one (1), linderinol (6), 4'-O-methylkaempferol 3-O-α-L-(4''-E-p-coumaroyl)rhamnoside (11) and kaempferol 3-O-α-L-(4''-Z-p-coumaroyl)rhamnoside (12) with eleven known compounds—3-epilistenolide D1 (2), 3-epilistenolide D2 (3), (3Z,4α,5β)-3-(dodec-11-ynylidene)-4-hydroxy-5-methylbutanolide (4), (3E,4β,5β)-3-(dodec-11-ynylidene)-4-hydroxy-5-methylbutanolide (5), matairesinol (7), syringaresinol (8), (+)-pinoresinol (9), salicifoliol (10), 4''-p-coumaroylafzelin (13), catechin (14) and epicatechin (15)—were first isolated from the aerial part of Lindera akoensis. Their structures were determined by detailed analysis of 1D- and 2D-NMR spectroscopic data. All of the compounds isolated from Lindera akoensis showed that in vitro anti-inflammatory activity decreases the LPS-stimulated production of nitric oxide (NO) in RAW 264.7 cell, with IC50 values of 4.1–413.8 µM.
Keywords: Chinese herb; Lindera akoensis; butanolide; lignans; flavonoids; anti-inflammatory
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Yang, C.-P.; Huang, G.-J.; Huang, H.-C.; Chen, Y.-C.; Chang, C.-I.; Wang, S.-Y.; Chang, H.-S.; Tseng, Y.-H.; Chien, S.-C.; Kuo, Y.-H. The Effect of the Aerial Part of Lindera akoensis on Lipopolysaccharides (LPS)-Induced Nitric Oxide Production in RAW264.7 Cells. Int. J. Mol. Sci. 2013, 14, 9168-9181.
Yang C-P, Huang G-J, Huang H-C, Chen Y-C, Chang C-I, Wang S-Y, Chang H-S, Tseng Y-H, Chien S-C, Kuo Y-H. The Effect of the Aerial Part of Lindera akoensis on Lipopolysaccharides (LPS)-Induced Nitric Oxide Production in RAW264.7 Cells. International Journal of Molecular Sciences. 2013; 14(5):9168-9181.
Yang, Chung-Ping; Huang, Guan-Jhong; Huang, Hui-Chi; Chen, Yu-Chang; Chang, Chi-I; Wang, Sheng-Yang; Chang, Hsun-Shuo; Tseng, Yen-Hsueh; Chien, Shih-Chang; Kuo, Yueh-Hsiung. 2013. "The Effect of the Aerial Part of Lindera akoensis on Lipopolysaccharides (LPS)-Induced Nitric Oxide Production in RAW264.7 Cells." Int. J. Mol. Sci. 14, no. 5: 9168-9181.