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Transcriptome Analysis of Thapsia laciniata Rouy Provides Insights into Terpenoid Biosynthesis and Diversity in Apiaceae
Department of Plant and Environmental Sciences, Faculty of Sciences, University of Copenhagen, Frederiksberg DK-1871, Denmark
Wine Science and Business, School of Agriculture Food and Wine, University of Adelaide, South Australia, SA 5064, Australia
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, AB T2N 1N4, Canada
* Author to whom correspondence should be addressed.
Received: 22 February 2013; in revised form: 21 March 2013 / Accepted: 17 April 2013 / Published: 25 April 2013
Abstract: Thapsia laciniata Rouy (Apiaceae) produces irregular and regular sesquiterpenoids with thapsane and guaiene carbon skeletons, as found in other Apiaceae species. A transcriptomic analysis utilizing Illumina next-generation sequencing enabled the identification of novel genes involved in the biosynthesis of terpenoids in Thapsia. From 66.78 million HQ paired-end reads obtained from T. laciniata roots, 64.58 million were assembled into 76,565 contigs (N50: 1261 bp). Seventeen contigs were annotated as terpene synthases and five of these were predicted to be sesquiterpene synthases. Of the 67 contigs annotated as cytochromes P450, 18 of these are part of the CYP71 clade that primarily performs hydroxylations of specialized metabolites. Three contigs annotated as aldehyde dehydrogenases grouped phylogenetically with the characterized ALDH1 from Artemisia annua and three contigs annotated as alcohol dehydrogenases grouped with the recently described ADH1 from A. annua. ALDH1 and ADH1 were characterized as part of the artemisinin biosynthesis. We have produced a comprehensive EST dataset for T. laciniata roots, which contains a large sample of the T. laciniata transcriptome. These transcriptome data provide the foundation for future research into the molecular basis for terpenoid biosynthesis in Thapsia and on the evolution of terpenoids in Apiaceae.
Keywords: Thapsia laciniata; guaianolides; thapsane; Apiaceae; sesquiterpenoids
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Drew, D.P.; Dueholm, B.; Weitzel, C.; Zhang, Y.; Sensen, C.W.; Simonsen, H.T. Transcriptome Analysis of Thapsia laciniata Rouy Provides Insights into Terpenoid Biosynthesis and Diversity in Apiaceae. Int. J. Mol. Sci. 2013, 14, 9080-9098.
Drew DP, Dueholm B, Weitzel C, Zhang Y, Sensen CW, Simonsen HT. Transcriptome Analysis of Thapsia laciniata Rouy Provides Insights into Terpenoid Biosynthesis and Diversity in Apiaceae. International Journal of Molecular Sciences. 2013; 14(5):9080-9098.
Drew, Damian P.; Dueholm, Bjørn; Weitzel, Corinna; Zhang, Ye; Sensen, Christoph W.; Simonsen, Henrik T. 2013. "Transcriptome Analysis of Thapsia laciniata Rouy Provides Insights into Terpenoid Biosynthesis and Diversity in Apiaceae." Int. J. Mol. Sci. 14, no. 5: 9080-9098.