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Improving Pharmaceutical Protein Production in Oryza sativa
Graduate School of Biotechnology and Bioengineering, Yuan Ze University, 135 Yuan-Tung Road, Taoyuan 32003, Taiwan
Department of Life Sciences, National Central University, 300, Jhongda Rd., Taoyuan 32001, Taiwan
* Author to whom correspondence should be addressed.
Received: 21 January 2013; in revised form: 14 April 2013 / Accepted: 15 April 2013 / Published: 24 April 2013
Abstract: Application of plant expression systems in the production of recombinant proteins has several advantages, such as low maintenance cost, absence of human pathogens, and possession of complex post-translational glycosylation capabilities. Plants have been successfully used to produce recombinant cytokines, vaccines, antibodies, and other proteins, and rice (Oryza sativa) is a potential plant used as recombinant protein expression system. After successful transformation, transgenic rice cells can be either regenerated into whole plants or grown as cell cultures that can be upscaled into bioreactors. This review summarizes recent advances in the production of different recombinant protein produced in rice and describes their production methods as well as methods to improve protein yield and quality. Glycosylation and its impact in plant development and protein production are discussed, and several methods of improving yield and quality that have not been incorporated in rice expression systems are also proposed. Finally, different bioreactor options are explored and their advantages are analyzed.
Keywords: bioreactor; glycosylation; inducible system; Oryza sativa; recombinant protein; suspension cells; transgenic plants
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MDPI and ACS Style
Kuo, Y.-C.; Tan, C.-C.; Ku, J.-T.; Hsu, W.-C.; Su, S.-C.; Lu, C.-A.; Huang, L.-F. Improving Pharmaceutical Protein Production in Oryza sativa. Int. J. Mol. Sci. 2013, 14, 8719-8739.
Kuo Y-C, Tan C-C, Ku J-T, Hsu W-C, Su S-C, Lu C-A, Huang L-F. Improving Pharmaceutical Protein Production in Oryza sativa. International Journal of Molecular Sciences. 2013; 14(5):8719-8739.
Kuo, Yu-Chieh; Tan, Chia-Chun; Ku, Jung-Ting; Hsu, Wei-Cho; Su, Sung-Chieh; Lu, Chung-An; Huang, Li-Fen. 2013. "Improving Pharmaceutical Protein Production in Oryza sativa." Int. J. Mol. Sci. 14, no. 5: 8719-8739.