Abstract: In this paper, we investigated the intra-species bacterial quorum sensing at the single cell level using a double droplet trapping system. Escherichia coli transformed to express the quorum sensing receptor protein, LasR, were encapsulated in microdroplets that were positioned adjacent to microdroplets containing the autoinducer, N-(3-oxododecanoyl)-L-homoserine lactone (OdDHL). Functional activation of the LasR protein by diffusion of the OdDHL across the droplet interface was measured by monitoring the expression of green fluorescent protein (GFP) from a LasR-dependent promoter. A threshold concentration of OdDHL was found to induce production of quorum-sensing associated GFP by E. coli. Additionally, we demonstrated that LasR-dependent activation of GFP expression was also initiated when the adjacent droplets contained single E. coli transformed with the OdDHL synthase gene, LasI, representing a simple quorum sensing circuit between two droplets.
Keywords: droplet-based microfluidics; bacterial quorum sensing; single cell
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Bai, Y.; Patil, S.N.; Bowden, S.D.; Poulter, S.; Pan, J.; Salmond, G.P.C.; Welch, M.; Huck, W.T.S.; Abell, C. Intra-Species Bacterial Quorum Sensing Studied at Single Cell Level in a Double Droplet Trapping System. Int. J. Mol. Sci. 2013, 14, 10570-10581.
Bai Y, Patil SN, Bowden SD, Poulter S, Pan J, Salmond GPC, Welch M, Huck WTS, Abell C. Intra-Species Bacterial Quorum Sensing Studied at Single Cell Level in a Double Droplet Trapping System. International Journal of Molecular Sciences. 2013; 14(5):10570-10581.
Bai, Yunpeng; Patil, Santoshkumar N.; Bowden, Steven D.; Poulter, Simon; Pan, Jie; Salmond, George P.C.; Welch, Martin; Huck, Wilhelm T.S.; Abell, Chris. 2013. "Intra-Species Bacterial Quorum Sensing Studied at Single Cell Level in a Double Droplet Trapping System." Int. J. Mol. Sci. 14, no. 5: 10570-10581.