Int. J. Mol. Sci. 2013, 14(5), 10570-10581; doi:10.3390/ijms140510570
Article

Intra-Species Bacterial Quorum Sensing Studied at Single Cell Level in a Double Droplet Trapping System

Received: 19 April 2013; in revised form: 9 May 2013 / Accepted: 10 May 2013 / Published: 21 May 2013
(This article belongs to the Special Issue Quorum Sensing Research in Microbial Systems)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: In this paper, we investigated the intra-species bacterial quorum sensing at the single cell level using a double droplet trapping system. Escherichia coli transformed to express the quorum sensing receptor protein, LasR, were encapsulated in microdroplets that were positioned adjacent to microdroplets containing the autoinducer, N-(3-oxododecanoyl)-L-homoserine lactone (OdDHL). Functional activation of the LasR protein by diffusion of the OdDHL across the droplet interface was measured by monitoring the expression of green fluorescent protein (GFP) from a LasR-dependent promoter. A threshold concentration of OdDHL was found to induce production of quorum-sensing associated GFP by E. coli. Additionally, we demonstrated that LasR-dependent activation of GFP expression was also initiated when the adjacent droplets contained single E. coli transformed with the OdDHL synthase gene, LasI, representing a simple quorum sensing circuit between two droplets.
Keywords: droplet-based microfluidics; bacterial quorum sensing; single cell
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MDPI and ACS Style

Bai, Y.; Patil, S.N.; Bowden, S.D.; Poulter, S.; Pan, J.; Salmond, G.P.C.; Welch, M.; Huck, W.T.S.; Abell, C. Intra-Species Bacterial Quorum Sensing Studied at Single Cell Level in a Double Droplet Trapping System. Int. J. Mol. Sci. 2013, 14, 10570-10581.

AMA Style

Bai Y, Patil SN, Bowden SD, Poulter S, Pan J, Salmond GPC, Welch M, Huck WTS, Abell C. Intra-Species Bacterial Quorum Sensing Studied at Single Cell Level in a Double Droplet Trapping System. International Journal of Molecular Sciences. 2013; 14(5):10570-10581.

Chicago/Turabian Style

Bai, Yunpeng; Patil, Santoshkumar N.; Bowden, Steven D.; Poulter, Simon; Pan, Jie; Salmond, George P.C.; Welch, Martin; Huck, Wilhelm T.S.; Abell, Chris. 2013. "Intra-Species Bacterial Quorum Sensing Studied at Single Cell Level in a Double Droplet Trapping System." Int. J. Mol. Sci. 14, no. 5: 10570-10581.

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