Int. J. Mol. Sci. 2013, 14(5), 10343-10354; doi:10.3390/ijms140510343
Article

Ischemic Preconditioning Protects against Spinal Cord Ischemia-Reperfusion Injury in Rabbits by Attenuating Blood Spinal Cord Barrier Disruption

1 Department of Anesthesiology, First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning, China 2 Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, Shenyang 110001, Liaoning, China
* Author to whom correspondence should be addressed.
Received: 25 February 2013; in revised form: 24 April 2013 / Accepted: 2 May 2013 / Published: 17 May 2013
(This article belongs to the Special Issue Neuroprotective Strategies 2014)
PDF Full-text Download PDF Full-Text [1992 KB, uploaded 17 May 2013 09:45 CEST]
Abstract: Ischemic preconditioning has been reported to protect against spinal cord ischemia-reperfusion (I-R) injury, but the underlying mechanisms are not fully understood. To investigate this, Japanese white rabbits underwent I-R (30 min aortic occlusion followed by reperfusion), ischemic preconditioning (three cycles of 5 min aortic occlusion plus 5 min reperfusion) followed by I-R, or sham surgery. At 4 and 24 h following reperfusion, neurological function was assessed using Tarlov scores, blood spinal cord barrier permeability was measured by Evan’s Blue extravasation, spinal cord edema was evaluated using the wet-dry method, and spinal cord expression of zonula occluden-1 (ZO-1), matrix metalloproteinase-9 (MMP-9), and tumor necrosis factor-α (TNF-α) were measured by Western blot and a real-time polymerase chain reaction. ZO-1 was also assessed using immunofluorescence. Spinal cord I-R injury reduced neurologic scores, and ischemic preconditioning treatment ameliorated this effect. Ischemic preconditioning inhibited I-R-induced increases in blood spinal cord barrier permeability and water content, increased ZO-1 mRNA and protein expression, and reduced MMP-9 and TNF-α mRNA and protein expression. These findings suggest that ischemic preconditioning attenuates the increase in blood spinal cord barrier permeability due to spinal cord I-R injury by preservation of tight junction protein ZO-1 and reducing MMP-9 and TNF-α expression.
Keywords: ischemic preconditioning; ischemia-reperfusion injury; spinal cord; blood spinal cord barrier

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Fang, B.; Li, X.-M.; Sun, X.-J.; Bao, N.-R.; Ren, X.-Y.; Lv, H.-W.; Ma, H. Ischemic Preconditioning Protects against Spinal Cord Ischemia-Reperfusion Injury in Rabbits by Attenuating Blood Spinal Cord Barrier Disruption. Int. J. Mol. Sci. 2013, 14, 10343-10354.

AMA Style

Fang B, Li X-M, Sun X-J, Bao N-R, Ren X-Y, Lv H-W, Ma H. Ischemic Preconditioning Protects against Spinal Cord Ischemia-Reperfusion Injury in Rabbits by Attenuating Blood Spinal Cord Barrier Disruption. International Journal of Molecular Sciences. 2013; 14(5):10343-10354.

Chicago/Turabian Style

Fang, Bo; Li, Xiao-Man; Sun, Xi-Jia; Bao, Na-Ren; Ren, Xiao-Yan; Lv, Huang-Wei; Ma, Hong. 2013. "Ischemic Preconditioning Protects against Spinal Cord Ischemia-Reperfusion Injury in Rabbits by Attenuating Blood Spinal Cord Barrier Disruption." Int. J. Mol. Sci. 14, no. 5: 10343-10354.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert