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An Exonuclease III Protection-Based Electrochemical Method for Estrogen Receptor Assay
Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China
Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China
* Authors to whom correspondence should be addressed.
Received: 6 March 2013; in revised form: 25 April 2013 / Accepted: 10 May 2013 / Published: 16 May 2013
Abstract: Estrogen receptor (ER), expressed in approximately 80% of primary breast cancer cells, has proven to be a valuable predictive factor of the disease. Herein, by making use of the specific binding of ER to its DNA response elements, we propose an Exonuclease III (Exo III) protection-based electrochemical method for detecting ER proteins. In this assay, the presence of ER can protect the duplex DNA molecules immobilized on an electrode surface from Exo III-catalyzed digestion, resulting in an increased electrochemical signal. Experimental results have revealed that the proposed method can allow the quantification of ER in the range of 0.5 to 100 nM with a satisfactory detection limit of 0.38 nM. Furthermore, since this approach can also be employed to detect ER directly in nuclear extracts, it may be of great use in biomedical applications in the future.
Keywords: estrogen receptor; estrogen response elements; Exonuclease III; electrochemical method
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MDPI and ACS Style
Zhu, S.; Cao, Y.; Xu, Y.; Yin, Y.; Li, G. An Exonuclease III Protection-Based Electrochemical Method for Estrogen Receptor Assay. Int. J. Mol. Sci. 2013, 14, 10298-10306.
Zhu S, Cao Y, Xu Y, Yin Y, Li G. An Exonuclease III Protection-Based Electrochemical Method for Estrogen Receptor Assay. International Journal of Molecular Sciences. 2013; 14(5):10298-10306.
Zhu, Sha; Cao, Ya; Xu, Yuanyuan; Yin, Yongmei; Li, Genxi. 2013. "An Exonuclease III Protection-Based Electrochemical Method for Estrogen Receptor Assay." Int. J. Mol. Sci. 14, no. 5: 10298-10306.