Int. J. Mol. Sci. 2013, 14(5), 10298-10306; doi:10.3390/ijms140510298
Article

An Exonuclease III Protection-Based Electrochemical Method for Estrogen Receptor Assay

1,2email, 3email, 2email, 1,* email and 2,3,* email
1 Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China 2 Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China 3 Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China
* Authors to whom correspondence should be addressed.
Received: 6 March 2013; in revised form: 25 April 2013 / Accepted: 10 May 2013 / Published: 16 May 2013
(This article belongs to the Special Issue Molecular Bases of Cancer Research)
PDF Full-text Download PDF Full-Text [421 KB, uploaded 16 May 2013 11:24 CEST]
Abstract: Estrogen receptor (ER), expressed in approximately 80% of primary breast cancer cells, has proven to be a valuable predictive factor of the disease. Herein, by making use of the specific binding of ER to its DNA response elements, we propose an Exonuclease III (Exo III) protection-based electrochemical method for detecting ER proteins. In this assay, the presence of ER can protect the duplex DNA molecules immobilized on an electrode surface from Exo III-catalyzed digestion, resulting in an increased electrochemical signal. Experimental results have revealed that the proposed method can allow the quantification of ER in the range of 0.5 to 100 nM with a satisfactory detection limit of 0.38 nM. Furthermore, since this approach can also be employed to detect ER directly in nuclear extracts, it may be of great use in biomedical applications in the future.
Keywords: estrogen receptor; estrogen response elements; Exonuclease III; electrochemical method

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Zhu, S.; Cao, Y.; Xu, Y.; Yin, Y.; Li, G. An Exonuclease III Protection-Based Electrochemical Method for Estrogen Receptor Assay. Int. J. Mol. Sci. 2013, 14, 10298-10306.

AMA Style

Zhu S, Cao Y, Xu Y, Yin Y, Li G. An Exonuclease III Protection-Based Electrochemical Method for Estrogen Receptor Assay. International Journal of Molecular Sciences. 2013; 14(5):10298-10306.

Chicago/Turabian Style

Zhu, Sha; Cao, Ya; Xu, Yuanyuan; Yin, Yongmei; Li, Genxi. 2013. "An Exonuclease III Protection-Based Electrochemical Method for Estrogen Receptor Assay." Int. J. Mol. Sci. 14, no. 5: 10298-10306.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert