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Int. J. Mol. Sci. 2013, 14(5), 10298-10306; doi:10.3390/ijms140510298
Article

An Exonuclease III Protection-Based Electrochemical Method for Estrogen Receptor Assay

1,2
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, 1,*  and 2,3,*
Received: 6 March 2013; in revised form: 25 April 2013 / Accepted: 10 May 2013 / Published: 16 May 2013
(This article belongs to the Special Issue Molecular Bases of Cancer Research)
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Abstract: Estrogen receptor (ER), expressed in approximately 80% of primary breast cancer cells, has proven to be a valuable predictive factor of the disease. Herein, by making use of the specific binding of ER to its DNA response elements, we propose an Exonuclease III (Exo III) protection-based electrochemical method for detecting ER proteins. In this assay, the presence of ER can protect the duplex DNA molecules immobilized on an electrode surface from Exo III-catalyzed digestion, resulting in an increased electrochemical signal. Experimental results have revealed that the proposed method can allow the quantification of ER in the range of 0.5 to 100 nM with a satisfactory detection limit of 0.38 nM. Furthermore, since this approach can also be employed to detect ER directly in nuclear extracts, it may be of great use in biomedical applications in the future.
Keywords: estrogen receptor; estrogen response elements; Exonuclease III; electrochemical method estrogen receptor; estrogen response elements; Exonuclease III; electrochemical method
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Zhu, S.; Cao, Y.; Xu, Y.; Yin, Y.; Li, G. An Exonuclease III Protection-Based Electrochemical Method for Estrogen Receptor Assay. Int. J. Mol. Sci. 2013, 14, 10298-10306.

AMA Style

Zhu S, Cao Y, Xu Y, Yin Y, Li G. An Exonuclease III Protection-Based Electrochemical Method for Estrogen Receptor Assay. International Journal of Molecular Sciences. 2013; 14(5):10298-10306.

Chicago/Turabian Style

Zhu, Sha; Cao, Ya; Xu, Yuanyuan; Yin, Yongmei; Li, Genxi. 2013. "An Exonuclease III Protection-Based Electrochemical Method for Estrogen Receptor Assay." Int. J. Mol. Sci. 14, no. 5: 10298-10306.


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