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Oxidative and Molecular Responses in Capsicum annuum L. after Hydrogen Peroxide, Salicylic Acid and Chitosan Foliar Applications
Biosystems Engineering Group, School of Engineering, Queretaro Autonomous University, C.U Cerro de las Campanas, S/N, colonia Las Campanas, CP 76010, Santiago de Querétaro, Querétaro, Mexico
Biotechnology Group, National Institute for Forestry, Agriculture and Livestock Research (INIFAP), Celaya-San Miguel de Allende, km 6, CP 38010, Celaya, Guanajuato, Mexico
* Author to whom correspondence should be addressed.
Received: 16 February 2013; in revised form: 24 April 2013 / Accepted: 2 May 2013 / Published: 15 May 2013
Abstract: Hydrogen peroxide (H2O2) is an important ROS molecule (Reactive oxygen species) that serves as a signal of oxidative stress and activation of signaling cascades as a result of the early response of the plant to biotic stress. This response can also be generated with the application of elicitors, stable molecules that induce the activation of transduction cascades and hormonal pathways, which trigger induced resistance to environmental stress. In this work, we evaluated the endogenous H2O2 production caused by salicylic acid (SA), chitosan (QN), and H2O2 elicitors in Capsicum annuum L. Hydrogen peroxide production after elicitation, catalase (CAT) and phenylalanine ammonia lyase (PAL) activities, as well as gene expression analysis of cat1, pal, and pathogenesis-related protein 1 (pr1) were determined. Our results displayed that 6.7 and 10 mM SA concentrations, and, 14 and 18 mM H2O2 concentrations, induced an endogenous H2O2 and gene expression. QN treatments induced the same responses in lesser proportion than the other two elicitors. Endogenous H2O2 production monitored during several days, showed results that could be an indicator for determining application opportunity uses in agriculture for maintaining plant alert systems against a stress.
Keywords: reactive oxygen species; oxidative stress; elicitors; oxidative stress dynamic; hydrogen peroxide; salicylic acid; chitosan; catalase; phenylalanine ammonia lyase
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Mejía-Teniente, L.; Durán-Flores, F.D.; Chapa-Oliver, A.M.; Torres-Pacheco, I.; Cruz-Hernández, A.; González-Chavira, M.M.; Ocampo-Velázquez, R.V.; Guevara-González, R.G. Oxidative and Molecular Responses in Capsicum annuum L. after Hydrogen Peroxide, Salicylic Acid and Chitosan Foliar Applications. Int. J. Mol. Sci. 2013, 14, 10178-10196.
Mejía-Teniente L, Durán-Flores FD, Chapa-Oliver AM, Torres-Pacheco I, Cruz-Hernández A, González-Chavira MM, Ocampo-Velázquez RV, Guevara-González RG. Oxidative and Molecular Responses in Capsicum annuum L. after Hydrogen Peroxide, Salicylic Acid and Chitosan Foliar Applications. International Journal of Molecular Sciences. 2013; 14(5):10178-10196.
Mejía-Teniente, Laura; Durán-Flores, Flor de Dalia; Chapa-Oliver, Angela M.; Torres-Pacheco, Irineo; Cruz-Hernández, Andrés; González-Chavira, Mario M.; Ocampo-Velázquez, Rosalía V.; Guevara-González, Ramón G. 2013. "Oxidative and Molecular Responses in Capsicum annuum L. after Hydrogen Peroxide, Salicylic Acid and Chitosan Foliar Applications." Int. J. Mol. Sci. 14, no. 5: 10178-10196.