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MiR-590-5P Inhibits Growth of HepG2 Cells via Decrease of S100A10 Expression and Inhibition of the Wnt Pathway
Department of Geraeology, Yancheng City No.1 People's Hospital, Yancheng 224001, China
Department of General Surgery, the First Affiliated Hospital of Soochow University, Suzhou 215006, China
Department of Medical Oncology, Yancheng City No.1 People's Hospital, Yancheng 224001, China
Department of General Surgery, Yancheng City No.1 People's Hospital, Yancheng 224001, China
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 19 February 2013; in revised form: 8 March 2013 / Accepted: 7 April 2013 / Published: 18 April 2013
Abstract: Hepatocellular carcinoma is one of the most common and lethal cancers worldwide, especially in developing countries. In the present study, we found that the expression of a microRNA, miR-590-5P, was down-regulated and S100A10 was up-regulated in six hepatocellular carcinoma cell lines. The reporter gene assay showed that overexpression of miR-590-5P effectively reduced the activity of luciferase expressed by a vector bearing the 3' untranslated region of S100A10 mRNA. Ectopic miR-590-5P overexpression mediated by lentiviral infection decreased expression of S100A10. Infection of Lv-miR-590-5P inhibited cell growth and induced cell cycle G1 arrest in HepG2 cells. In addition, miR-590-5P expression suppressed the expression of Wnt5a, cMyc and cyclin D1, and increased the phosphorylation of β-catenin and expression of Caspase 3, which may contribute to the inhibitory effect of miR-590-5P on cell growth. Taken together, our data suggest that down-regulation of miR-590-5P is involved in hepatocellular carcinoma and the restoration of miR-590-5P can impair the growth of cancer cells, suggesting that miR-590-5P may be a potential target molecule for the therapy of hepatocellular carcinoma.
Keywords: miR-590-5P; S100A10; hepatocellular carcinoma; Wnt pathway; lentiviral system; reporter gene
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Shan, X.; Miao, Y.; Fan, R.; Qian, H.; Chen, P.; Liu, H.; Yan, X.; Li, J.; Zhou, F. MiR-590-5P Inhibits Growth of HepG2 Cells via Decrease of S100A10 Expression and Inhibition of the Wnt Pathway. Int. J. Mol. Sci. 2013, 14, 8556-8569.
Shan X, Miao Y, Fan R, Qian H, Chen P, Liu H, Yan X, Li J, Zhou F. MiR-590-5P Inhibits Growth of HepG2 Cells via Decrease of S100A10 Expression and Inhibition of the Wnt Pathway. International Journal of Molecular Sciences. 2013; 14(4):8556-8569.
Shan, Xiangxiang; Miao, Yufeng; Fan, Rengen; Qian, Haixin; Chen, Ping; Liu, Hongqi; Yan, Xiaomei; Li, Jianping; Zhou, Fen. 2013. "MiR-590-5P Inhibits Growth of HepG2 Cells via Decrease of S100A10 Expression and Inhibition of the Wnt Pathway." Int. J. Mol. Sci. 14, no. 4: 8556-8569.