Next Article in Journal
Identifying Differentially Expressed Genes in Pollen from Self-Incompatible “Wuzishatangju” and Self-Compatible “Shatangju” Mandarins
Next Article in Special Issue
Phospholipids at the Interface: Current Trends and Challenges
Previous Article in Journal
Antibacterial Activity of the Alkaloid-Enriched Extract from Prosopis juliflora Pods and Its Influence on in Vitro Ruminal Digestion
Previous Article in Special Issue
Phospholipid Membrane Protection by Sugar Molecules during Dehydration—Insights into Molecular Mechanisms Using Scattering Techniques
Int. J. Mol. Sci. 2013, 14(4), 8517-8537; doi:10.3390/ijms14048517
Review

Crosstalk between DnaA Protein, the Initiator of Escherichia coli Chromosomal Replication, and Acidic Phospholipids Present in Bacterial Membranes

1,* , 2
,
1
,
1
 and
1,3
1 Department of Biochemistry and Molecular & Cellular Biology Georgetown University Medical Center, Washington, DC 20007, USA 2 Jet Propulsion Laboratory, California Institute of Technology, M/S: 183-426, 4800 Oak Grove Drive, Pasadena, CA 91109, USA 3 Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20007, USA
* Author to whom correspondence should be addressed.
Received: 21 January 2013 / Revised: 3 April 2013 / Accepted: 6 April 2013 / Published: 17 April 2013
(This article belongs to the Special Issue Phospholipids: Molecular Sciences 2012)
View Full-Text   |   Download PDF [773 KB, 19 June 2014; original version 19 June 2014]   |   Browse Figures

Abstract

Anionic (i.e., acidic) phospholipids such as phosphotidylglycerol (PG) and cardiolipin (CL), participate in several cellular functions. Here we review intriguing in vitro and in vivo evidence that suggest emergent roles for acidic phospholipids in regulating DnaA protein-mediated initiation of Escherichia coli chromosomal replication. In vitro acidic phospholipids in a fluid bilayer promote the conversion of inactive ADP-DnaA to replicatively proficient ATP-DnaA, yet both PG and CL also can inhibit the DNA-binding activity of DnaA protein. We discuss how cellular acidic phospholipids may positively and negatively influence the initiation activity of DnaA protein to help assure chromosomal replication occurs once, but only once, per cell-cycle. Fluorescence microscopy has revealed that PG and CL exist in domains located at the cell poles and mid-cell, and several studies link membrane curvature with sub-cellular localization of various integral and peripheral membrane proteins. E. coli DnaA itself is found at the cell membrane and forms helical structures along the longitudinal axis of the cell. We propose that there is cross-talk between acidic phospholipids in the bacterial membrane and DnaA protein as a means to help control the spatial and temporal regulation of chromosomal replication in bacteria.
Keywords: acidic phospholipids; DnaA protein; chromosomal replication; Escherichia coli acidic phospholipids; DnaA protein; chromosomal replication; Escherichia coli
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote
MDPI and ACS Style

Saxena, R.; Fingland, N.; Patil, D.; Sharma, A.K.; Crooke, E. Crosstalk between DnaA Protein, the Initiator of Escherichia coli Chromosomal Replication, and Acidic Phospholipids Present in Bacterial Membranes. Int. J. Mol. Sci. 2013, 14, 8517-8537.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

Cited By

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert