Int. J. Mol. Sci. 2013, 14(4), 8517-8537; doi:10.3390/ijms14048517
Review

Crosstalk between DnaA Protein, the Initiator of Escherichia coli Chromosomal Replication, and Acidic Phospholipids Present in Bacterial Membranes

1 Department of Biochemistry and Molecular & Cellular Biology Georgetown University Medical Center, Washington, DC 20007, USA 2 Jet Propulsion Laboratory, California Institute of Technology, M/S: 183-426, 4800 Oak Grove Drive, Pasadena, CA 91109, USA 3 Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20007, USA
* Author to whom correspondence should be addressed.
Received: 21 January 2013; in revised form: 3 April 2013 / Accepted: 6 April 2013 / Published: 17 April 2013
(This article belongs to the Special Issue Phospholipids: Molecular Sciences 2012)
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Abstract: Anionic (i.e., acidic) phospholipids such as phosphotidylglycerol (PG) and cardiolipin (CL), participate in several cellular functions. Here we review intriguing in vitro and in vivo evidence that suggest emergent roles for acidic phospholipids in regulating DnaA protein-mediated initiation of Escherichia coli chromosomal replication. In vitro acidic phospholipids in a fluid bilayer promote the conversion of inactive ADP-DnaA to replicatively proficient ATP-DnaA, yet both PG and CL also can inhibit the DNA-binding activity of DnaA protein. We discuss how cellular acidic phospholipids may positively and negatively influence the initiation activity of DnaA protein to help assure chromosomal replication occurs once, but only once, per cell-cycle. Fluorescence microscopy has revealed that PG and CL exist in domains located at the cell poles and mid-cell, and several studies link membrane curvature with sub-cellular localization of various integral and peripheral membrane proteins. E. coli DnaA itself is found at the cell membrane and forms helical structures along the longitudinal axis of the cell. We propose that there is cross-talk between acidic phospholipids in the bacterial membrane and DnaA protein as a means to help control the spatial and temporal regulation of chromosomal replication in bacteria.
Keywords: acidic phospholipids; DnaA protein; chromosomal replication; Escherichia coli

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MDPI and ACS Style

Saxena, R.; Fingland, N.; Patil, D.; Sharma, A.K.; Crooke, E. Crosstalk between DnaA Protein, the Initiator of Escherichia coli Chromosomal Replication, and Acidic Phospholipids Present in Bacterial Membranes. Int. J. Mol. Sci. 2013, 14, 8517-8537.

AMA Style

Saxena R, Fingland N, Patil D, Sharma AK, Crooke E. Crosstalk between DnaA Protein, the Initiator of Escherichia coli Chromosomal Replication, and Acidic Phospholipids Present in Bacterial Membranes. International Journal of Molecular Sciences. 2013; 14(4):8517-8537.

Chicago/Turabian Style

Saxena, Rahul; Fingland, Nicholas; Patil, Digvijay; Sharma, Anjali K.; Crooke, Elliott. 2013. "Crosstalk between DnaA Protein, the Initiator of Escherichia coli Chromosomal Replication, and Acidic Phospholipids Present in Bacterial Membranes." Int. J. Mol. Sci. 14, no. 4: 8517-8537.

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