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Structure and Conformational Dynamics of DMPC/Dicationic Surfactant and DMPC/Dicationic Surfactant/DNA Systems
Department of Macromolecular Physics, Adam Mickiewicz University, Umultowska 85, Poznań 61-614, Poland
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 28 January 2013; in revised form: 21 March 2013 / Accepted: 29 March 2013 / Published: 9 April 2013
Abstract: Amphiphilic dicationic surfactants, known as gemini surfactants, are currently studied for gene delivery purposes. The gemini surfactant molecule is composed of two hydrophilic “head” groups attached to hydrophobic chains and connected via molecular linker between them. The influence of different concentrations of 1,5-bis (1-imidazolilo-3-decyloxymethyl) pentane chloride (gemini surfactant) on the thermotropic phase behaviour of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) bilayers with and without the presence of DNA was investigated using Fourier transformed infrared (FTIR) and circular dichroism (CD) spectroscopies, small angle scattering of synchrotron radiation and differential scanning calorimetry. With increasing concentration of surfactant in DMPC/DNA systems, a disappearance of pretransition and a decrease in the main phase transition enthalpy and temperature were observed. The increasing intensity of diffraction peaks as a function of surfactant concentration also clearly shows the ability of the surfactant to promote the organisation of lipid bilayers in the multilayer lamellar phase.
Keywords: phospholipids; dicationic surfactant; gemini surfactant; DNA; DNA-lipid interactions; DNA-surfactant interactions
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Pietralik, Z.; Krzysztoń, R.; Kida, W.; Andrzejewska, W.; Kozak, M. Structure and Conformational Dynamics of DMPC/Dicationic Surfactant and DMPC/Dicationic Surfactant/DNA Systems. Int. J. Mol. Sci. 2013, 14, 7642-7659.
Pietralik Z, Krzysztoń R, Kida W, Andrzejewska W, Kozak M. Structure and Conformational Dynamics of DMPC/Dicationic Surfactant and DMPC/Dicationic Surfactant/DNA Systems. International Journal of Molecular Sciences. 2013; 14(4):7642-7659.
Pietralik, Zuzanna; Krzysztoń, Rafał; Kida, Wojciech; Andrzejewska, Weronika; Kozak, Maciej. 2013. "Structure and Conformational Dynamics of DMPC/Dicationic Surfactant and DMPC/Dicationic Surfactant/DNA Systems." Int. J. Mol. Sci. 14, no. 4: 7642-7659.