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Int. J. Mol. Sci. 2013, 14(4), 6556-6570; doi:10.3390/ijms14046556
Article

Noninvasive Metabolomic Profiling of Human Embryo Culture Media Using a Simple Spectroscopy Adjunct to Morphology for Embryo Assessment in in Vitro Fertilization (IVF)

1,†
,
1,†
,
2
,
1
,
1,* , 2
 and
2,*
1 Reproductive Medical Center, Renmin Hospital of Wuhan University, JieFang Road 238, Wuhan 430060, China 2 College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072, China These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 23 December 2012 / Revised: 27 February 2013 / Accepted: 7 March 2013 / Published: 25 March 2013
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Abstract

Embryo quality is crucial to the outcome of in vitro fertilization (IVF); however, the ability to precisely distinguish the embryos with higher reproductive potential from others is poor. Morphologic evaluation used to play an important role in assessing embryo quality, but it is somewhat subjective. The culture medium is the immediate environment of the embryos in vitro, and a change of the substances in the culture medium is possibly related to the embryo quality. Thus, the present study aims to determine whether metabolomic profiling of the culture medium using Raman spectroscopy adjunct to morphology correlates with the reproductive potential of embryos in IVF and, thus, to look for a new method of assessing embryo quality. Fifty seven spent media samples were detected by Raman spectroscopy. Combined with embryo morphology scores, we found that embryos in culture media with less than 0.012 of sodium pyruvate and more than −0.00085 phenylalanine have a high reproductive potential, with up to 85.7% accuracy compared with clinical pregnancy. So, sodium pyruvate and phenylalanine in culture medium play an important role in the development of the embryo. Raman spectroscopy is an important tool that provides a new and accurate assessment of higher quality embryos.
Keywords: metabolomics; Raman spectroscopy; culture media; IVF metabolomics; Raman spectroscopy; culture media; IVF
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Zhao, Q.; Yin, T.; Peng, J.; Zou, Y.; Yang, J.; Shen, A.; Hu, J. Noninvasive Metabolomic Profiling of Human Embryo Culture Media Using a Simple Spectroscopy Adjunct to Morphology for Embryo Assessment in in Vitro Fertilization (IVF). Int. J. Mol. Sci. 2013, 14, 6556-6570.

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