Ribosomal Initiation Complex Assembly within the Wild-Strain of Coxsackievirus B3 and Live-Attenuated Sabin3-like IRESes during the Initiation of Translation
Abstract
:1. Introduction
2. Results
2.1. Amplification and Cloning of the IRES
2.2. RNA and Fluorescent Labeling
2.3. Initiation Complexes Assembly
3. Discussion
4. Experimental Section
4.1. Virus
4.2. Plasmids and Cloning Conditions
4.3. Bacterial Strains
4.4. Viral RNA Extraction
4.5. Reverse Transcription (RT)
4.6. Polymerase Chain Reaction
4.7. Cloning of the IRES in the pUC19 Vector
4.8. Screening of Recombinant Clones
4.9. RNA and in Vitro Transcription
4.10. Periodate Oxidation and Fluorophore Coupling
4.11. Mobility of Initiation Complexes during Sucrose Density Gradients Centrifugation
5. Conclusion
Acknowledgments
Conflict of Interest
References
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Souii, A.; M'hadheb-Gharbi, M.B.; Sargueil, B.; Brossard, A.; Chamond, N.; Aouni, M.; Gharbi, J. Ribosomal Initiation Complex Assembly within the Wild-Strain of Coxsackievirus B3 and Live-Attenuated Sabin3-like IRESes during the Initiation of Translation. Int. J. Mol. Sci. 2013, 14, 4400-4418. https://doi.org/10.3390/ijms14034400
Souii A, M'hadheb-Gharbi MB, Sargueil B, Brossard A, Chamond N, Aouni M, Gharbi J. Ribosomal Initiation Complex Assembly within the Wild-Strain of Coxsackievirus B3 and Live-Attenuated Sabin3-like IRESes during the Initiation of Translation. International Journal of Molecular Sciences. 2013; 14(3):4400-4418. https://doi.org/10.3390/ijms14034400
Chicago/Turabian StyleSouii, Amira, Manel Ben M'hadheb-Gharbi, Bruno Sargueil, Audrey Brossard, Nathalie Chamond, Mahjoub Aouni, and Jawhar Gharbi. 2013. "Ribosomal Initiation Complex Assembly within the Wild-Strain of Coxsackievirus B3 and Live-Attenuated Sabin3-like IRESes during the Initiation of Translation" International Journal of Molecular Sciences 14, no. 3: 4400-4418. https://doi.org/10.3390/ijms14034400