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Int. J. Mol. Sci. 2013, 14(2), 4326-4333; doi:10.3390/ijms14024326
Article

Molecular Typing of Mastitis-Causing Staphylococcus aureus Isolated from Heifers and Cows

1,†,* , 1
, 1
, 2
, 1
 and 1,†,*
Received: 22 November 2012; in revised form: 31 January 2013 / Accepted: 5 February 2013 / Published: 21 February 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Staphylococcus aureus is among the main etiologic agents of bovine mastitis. A total of 83 isolates of S. aureus from mammary glands of primiparous heifers were collected in the prepartum, calving and during lactation. For lactating cows, a total of 27 isolates of S. aureus from mammary glands were collected during lactation. The samples were taken in two dairy farms located in Sao Paulo State, Brazil. The highest frequency of S. aureus isolation in heifers was at the end of lactation. Strains were typified through Pulsed-field gel electrophoresis (PFGE) and grouped according to patterns of restriction enzyme SmaI. PFGE generated seven clonal profiles that were grouped into three different lineages, with the LA lineage being predominant and identified in heifers, as well as in the cows from the two regions studied. It was concluded that the cows showed a significant source of dispersion of S. aureus. At the first lactation the heifers were infected by the same clonal profiles of S. aureus which were isolated from multiparous lactating cows. The heifers were infected during milking over the months of lactation.
Keywords: epidemiology; heifers; PFGE epidemiology; heifers; PFGE
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Castelani, L.; Santos, A.F.S.; dos Santos Miranda, M.; Zafalon, L.F.; Pozzi, C.R.; Arcaro, J.R.P. Molecular Typing of Mastitis-Causing Staphylococcus aureus Isolated from Heifers and Cows. Int. J. Mol. Sci. 2013, 14, 4326-4333.

AMA Style

Castelani L, Santos AFS, dos Santos Miranda M, Zafalon LF, Pozzi CR, Arcaro JRP. Molecular Typing of Mastitis-Causing Staphylococcus aureus Isolated from Heifers and Cows. International Journal of Molecular Sciences. 2013; 14(2):4326-4333.

Chicago/Turabian Style

Castelani, Lívia; Santos, Aline F.S.; dos Santos Miranda, Mariana; Zafalon, Luiz F.; Pozzi, Claudia R.; Arcaro, Juliana R.P. 2013. "Molecular Typing of Mastitis-Causing Staphylococcus aureus Isolated from Heifers and Cows." Int. J. Mol. Sci. 14, no. 2: 4326-4333.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert