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Int. J. Mol. Sci. 2013, 14(2), 3718-3733; doi:10.3390/ijms14023718
Article

Exercise Therapy Downregulates the Overexpression of TLR4, TLR2, MyD88 and NF-κB after Cerebral Ischemia in Rats

* ,
 and
Department of Rehabilitation Medicine, the First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning, China These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 28 September 2012 / Revised: 6 December 2012 / Accepted: 28 January 2013 / Published: 7 February 2013
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Abstract

Toll-like receptor 2 (TLR2) and Toll-like receptor 4 (TLR4) are considered to mediate the inflammatory reaction of cerebral ischemia injury, and exercise can inhibit the activity of the Toll-like receptor signaling pathway in the peripheral blood of humans. Although physical exercise has been demonstrated to be neuroprotective in both clinical and laboratory settings, the underlying mechanism remains unclear. To clarify this critical issue, this study investigated the effects of treadmill training on the recovery of neurological function and the expression of TLR2 and TLR4 and their main downstream targets, nuclear factor-kappaB (NF-κB) and myeloid differentiation factor 88 (MyD88), in the ischemic rat brain after middle cerebral artery occlusion-reperfusion (MCAo/R). Rats were divided into seven groups: sham control without MCAo/R and five, nine and 16 days post-ischemic exercise or non-exercise. The neurological function and infarct volume were measured, and reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were used to detect the expression of TLR2, TLR4, NF-κB and MyD88 in ischemic brain tissue. The results indicated that treadmill training promoted functional recovery and reduced the overexpression of TLR2, TLR4, NF-κB and MyD88 in rat brain tissue after ischemia, a finding that may have implications for understanding the mechanism of exercise therapy after brain ischemia and indicating new therapeutic strategies for the pharmacological modulation of TLR signaling.
Keywords: neuroprotection; cerebral ischemia; TLR2; TLR4; exercise; NF-κB; MyD88 neuroprotection; cerebral ischemia; TLR2; TLR4; exercise; NF-κB; MyD88
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Ma, Y.; He, M.; Qiang, L. Exercise Therapy Downregulates the Overexpression of TLR4, TLR2, MyD88 and NF-κB after Cerebral Ischemia in Rats. Int. J. Mol. Sci. 2013, 14, 3718-3733.

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