Int. J. Mol. Sci. 2013, 14(2), 2684-2706; doi:10.3390/ijms14022684
Article

Effects of Heme Oxygenase-1 Upregulation on Blood Pressure and Cardiac Function in an Animal Model of Hypertensive Myocardial Infarction

1,2,†email, 1,†email, 1email, 1email, 1email, 1email, 3email and 1,* email
Received: 19 November 2012; in revised form: 6 January 2013 / Accepted: 21 January 2013 / Published: 28 January 2013
(This article belongs to the Special Issue Enzyme Optimization and Immobilization)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: In this study, we evaluate the effect of HO-1 upregulation on blood pressure and cardiac function in the new model of infarct spontaneous hypertensive rats (ISHR). Male spontaneous hypertensive rats (SHR) at 13 weeks (n = 40) and age-matched male Wistar (WT) rats (n = 20) were divided into six groups: WT (sham + normal saline (NS)), WT (sham + Co(III) Protoporphyrin IX Chloride (CoPP)), SHR (myocardial infarction (MI) + NS), SHR (MI + CoPP), SHR (MI + CoPP + Tin Mesoporphyrin IX Dichloride (SnMP)), SHR (sham + NS); CoPP 4.5 mg/kg, SnMP 15 mg/kg, for six weeks, one/week, i.p., n = 10/group. At the sixth week, echocardiography (UCG) and hemodynamics were performed. Then, blood samples and heart tissue were collected. Copp treatment in the SHR (MI + CoPP) group lowered blood pressure, decreased infarcted area, restored cardiac function (left ventricular ejection fraction (LVEF), left ventricular fraction shortening (LVFS), +dp/dtmax, (−dp/dtmax)/left ventricular systolic pressure (LVSP)), inhibited cardiac hypertrophy and ventricular enlargement (downregulating left ventricular end-systolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD) and heart weight/body weight (HW/BW)), lowered serum CRP, IL-6 and Glu levels and increased serum TB, NO and PGI2 levels. Western blot and immunohistochemistry showed that HO-1 expression was elevated in the SHR (MI + CoPP) group, while co-administration with SnMP suppressed the benefit functions mentioned above. In conclusion, HO-1 upregulation can lower blood pressure and improve post-infarct cardiac function in the ISHR model. These functions may be involved in the inhibition of inflammation and the ventricular remodeling process and in the amelioration of glucose metabolism and endothelial dysfunction.
Keywords: hypertension; myocardial infarction; heme oxygenase; bilirubin
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MDPI and ACS Style

Chen, T.-M.; Li, J.; Liu, L.; Fan, L.; Li, X.-Y.; Wang, Y.-T.; Abraham, N.G.; Cao, J. Effects of Heme Oxygenase-1 Upregulation on Blood Pressure and Cardiac Function in an Animal Model of Hypertensive Myocardial Infarction. Int. J. Mol. Sci. 2013, 14, 2684-2706.

AMA Style

Chen T-M, Li J, Liu L, Fan L, Li X-Y, Wang Y-T, Abraham NG, Cao J. Effects of Heme Oxygenase-1 Upregulation on Blood Pressure and Cardiac Function in an Animal Model of Hypertensive Myocardial Infarction. International Journal of Molecular Sciences. 2013; 14(2):2684-2706.

Chicago/Turabian Style

Chen, Tian-meng; Li, Jian; Liu, Lin; Fan, Li; Li, Xiao-ying; Wang, Yu-tang; Abraham, Nader G.; Cao, Jian. 2013. "Effects of Heme Oxygenase-1 Upregulation on Blood Pressure and Cardiac Function in an Animal Model of Hypertensive Myocardial Infarction." Int. J. Mol. Sci. 14, no. 2: 2684-2706.


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