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Identification and Characterization of Seven Glutathione S-Transferase Genes from Citrus Red Mite, Panonychus citri (McGregor)
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 10 September 2013; in revised form: 21 November 2013 / Accepted: 22 November 2013 / Published: 13 December 2013
Abstract: The citrus red mite, Panonychus citri (McGregor), is a global citrus pest, and has developed severe resistance to several types of acaricides. However, the molecular mechanisms of resistance in this mite remain unknown. In this study, seven full-length cDNAs encoding glutathione S-transferases (GSTs) genes were identified and characterized in P. citri. The effects of pyridaben and fenpropathrin exposure on the expression of these genes were also investigated. Phylogenetic analysis revealed that the seven GSTs genes in P. citri cloned in this study belong to three different cytosolic classes, including four in mu, two in delta and one in zeta. Among these seven GSTs genes, the relative expression level of PcGSTm1 was significantly higher in adult than in the other life stages (egg, larvae and nymph). Compared with the control, the mRNA levels of the seven GST genes did not change significantly following exposure to pyridaben at LC10. However, RT-qPCR results showed that, when exposed to LC10 of fenpropathrin, six GSTs gene (PcGSTm1, PcGSTm3, PcGSTm4, PcGSTd1, PcGSTd2 and PcGSTz1) transcripts increased in a time-dependent manner. This is the first insight into the molecular characteristics of GSTs gene cDNAs in P. citri. The elevated GSTs gene transcripts following exposure to fenpropathrin might be one of the mechanisms involved in detoxification of this acaricide.
Keywords: Panonychus citri; glutathione S-transferase; gene expression; pyridaben; fenpropathrin
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MDPI and ACS Style
Liao, C.-Y.; Zhang, K.; Niu, J.-Z.; Ding, T.-B.; Zhong, R.; Xia, W.-K.; Dou, W.; Wang, J.-J. Identification and Characterization of Seven Glutathione S-Transferase Genes from Citrus Red Mite, Panonychus citri (McGregor). Int. J. Mol. Sci. 2013, 14, 24255-24270.
Liao C-Y, Zhang K, Niu J-Z, Ding T-B, Zhong R, Xia W-K, Dou W, Wang J-J. Identification and Characterization of Seven Glutathione S-Transferase Genes from Citrus Red Mite, Panonychus citri (McGregor). International Journal of Molecular Sciences. 2013; 14(12):24255-24270.
Liao, Chong-Yu; Zhang, Kun; Niu, Jin-Zhi; Ding, Tian-Bo; Zhong, Rui; Xia, Wen-Kai; Dou, Wei; Wang, Jin-Jun. 2013. "Identification and Characterization of Seven Glutathione S-Transferase Genes from Citrus Red Mite, Panonychus citri (McGregor)." Int. J. Mol. Sci. 14, no. 12: 24255-24270.