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Genome-Wide Analysis of Respiratory Burst Oxidase Homologs in Grape (Vitis vinifera L.)
State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China
Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China
* Author to whom correspondence should be addressed.
Received: 2 November 2013; in revised form: 1 December 2013 / Accepted: 6 December 2013 / Published: 12 December 2013
Abstract: Plant respiratory burst oxidase homolog (rboh) genes appear to play crucial roles in plant development, defense reactions and hormone signaling. In this study, a total of seven rboh genes from grape were identified and characterized. Genomic structure and predicted protein sequence analysis indicated that the sequences of plant rboh genes are highly conserved. Synteny analysis demonstrated that several Vvrboh genes were found in corresponding syntenic blocks of Arabidopsis, suggesting that these genes arose before the divergence of the respective lineages. The expression pattern of Vvrboh genes in different tissues was assessed by qRT-PCR and two were constitutively expressed in all tissues tested. The expression profiles were similarly analyzed following exposure to various stresses and hormone treatments. It was shown that the expression levels of VvrbohA, VvrbohB and VvrbohC1 were significantly increased by salt and drought treatments. VvrbohB, VvrbohC2, and VvrbohD exhibited a dramatic up-regulation after powdery mildew (Uncinula necator (Schw.) Burr.) inoculation, while VvrbohH was down-regulated. Finally, salicylic acid treatment strongly stimulated the expression of VvrbohD and VvrbohH, while abscisic acid treatment induced the expression of VvrbohB and VvrbohH. These results demonstrate that the expression patterns of grape rboh genes exhibit diverse and complex stress-response expression signatures.
Keywords: reactive oxygen species; synteny analysis; phylogenetic analysis; gene expression
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MDPI and ACS Style
Cheng, C.; Xu, X.; Gao, M.; Li, J.; Guo, C.; Song, J.; Wang, X. Genome-Wide Analysis of Respiratory Burst Oxidase Homologs in Grape (Vitis vinifera L.). Int. J. Mol. Sci. 2013, 14, 24169-24186.
Cheng C, Xu X, Gao M, Li J, Guo C, Song J, Wang X. Genome-Wide Analysis of Respiratory Burst Oxidase Homologs in Grape (Vitis vinifera L.). International Journal of Molecular Sciences. 2013; 14(12):24169-24186.
Cheng, Chenxia; Xu, Xiaozhao; Gao, Min; Li, Jun; Guo, Chunlei; Song, Junyang; Wang, Xiping. 2013. "Genome-Wide Analysis of Respiratory Burst Oxidase Homologs in Grape (Vitis vinifera L.)." Int. J. Mol. Sci. 14, no. 12: 24169-24186.