Antiepileptic Potential of Matrine via Regulation the Levels of Gamma-Aminobutyric Acid and Glutamic Acid in the Brain
Abstract
:1. Introduction
2. Results and Discussion
2.1. Result of the Maximal Electroshock Test
2.2. Result of Pentylenetetrazole-Induced Seizure Test
2.3. Result of Pentobarbital Sodium-Induced Anaesthetizing Time in Mice
2.4. Results of Mouse Locomotor Activity Tests
2.5. Results of the Contents of Asp, GABA, Glu, and Gly in Mouse Brain
2.6. Effect of Matrine on Expressions of GAD 65, GABAA, GABAB in the Brain of Epileptic Rats
2.7. Discussion
3. Experimental Section
3.1. Animals
3.2. Drugs and Chemicals
3.3. Protocols
3.4. Maximal Electroshock (MES)-Induced Seizures in Mice
3.5. Pentylenetetrazole (PTZ)-Induced Convulsion in Mice
3.6. Pentobarbital Sodium-Induced Anaesthetizing Time in Mice
3.7. Measurement of Locomotor Activity
3.8. Measurement of the Contents of Asp, GABA, Glu, and Gly in Brain
3.9. Chronic Epileptic Rat Seizure Induced by PTZ and Western Blotting
3.10. Statistical Analysis
4. Conclusions
Conflicts of Interest
References
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Treatment | Dose | Convulsions (n) | Inhibition (%) | Tonic-clonus (%) | Death (n) | Mortality (%) |
---|---|---|---|---|---|---|
Control | 20 mL/kg | 15 | 0 | 100 | 3 | 20 |
Positive | 20 mL/kg | 0 | 100 ** | 0 ** | 0 | 0 |
Matrine | 7.5 mg/kg | 12 | 20 | 73.3 | 2 | 13.3 |
15 mg/kg | 8 | 46.7 * | 40 ** | 0 | 0 | |
30 mg/kg | 4 | 73.3 ** | 20 ** | 0 | 0 | |
60 mg/kg | 0 | 100 ** | 0 ** | 0 | 0 |
Treatment | Dose | Convulsions (n) | Inhibition (%) | Death (n) | Mortality (%) | ||
---|---|---|---|---|---|---|---|
IV–V | V | IV–V | V | ||||
Control | 20 mL/kg | 15 | 15 | 0 | 0 | 15 | 100 |
Positive | 4 mL/kg | 0 | 0 | 100 ** | 100 ** | 0 | 0 ** |
Matrine | 7.5 mg/kg | 8 | 7 | 46.7 * | 53.3 * | 9 | 60 * |
15 mg/kg | 7 | 5 | 53.3 ** | 66.7 ** | 6 | 40 * | |
30 mg/kg | 5 | 3 | 66.7 ** | 80 ** | 4 | 26.6 ** | |
60 mg/kg | 4 | 2 | 73.3 ** | 86.6 ** | 2 | 13.3 ** |
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Xiang, J.; Jiang, Y. Antiepileptic Potential of Matrine via Regulation the Levels of Gamma-Aminobutyric Acid and Glutamic Acid in the Brain. Int. J. Mol. Sci. 2013, 14, 23751-23761. https://doi.org/10.3390/ijms141223751
Xiang J, Jiang Y. Antiepileptic Potential of Matrine via Regulation the Levels of Gamma-Aminobutyric Acid and Glutamic Acid in the Brain. International Journal of Molecular Sciences. 2013; 14(12):23751-23761. https://doi.org/10.3390/ijms141223751
Chicago/Turabian StyleXiang, Jun, and Yugang Jiang. 2013. "Antiepileptic Potential of Matrine via Regulation the Levels of Gamma-Aminobutyric Acid and Glutamic Acid in the Brain" International Journal of Molecular Sciences 14, no. 12: 23751-23761. https://doi.org/10.3390/ijms141223751