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Int. J. Mol. Sci. 2013, 14(12), 23289-23296; doi:10.3390/ijms141223289
Technical Note

An Exploratory Evaluation of Tyrosine Hydroxylase Inhibition in Planaria as a Model for Parkinsonism

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Received: 11 October 2013; in revised form: 18 November 2013 / Accepted: 19 November 2013 / Published: 26 November 2013
(This article belongs to the Special Issue Neuroprotective Strategies 2014)
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Abstract: Planaria are the simplest organisms with bilateral symmetry and a central nervous system (CNS) with cephalization; therefore, they could be useful as model organisms to investigate mechanistic aspects of parkinsonism and to screen potential therapeutic agents. Taking advantage of the organism’s anti-tropism towards light, we measured a significantly reduced locomotor velocity in planaria after exposure to 3-iodo-L-tyrosine, an inhibitor of tyrosine hydroxylase that is an enzyme catalyzing the first and rate-limiting step in the biosynthesis of catecholamines. A simple semi-automatic assay using videotaped experiments and subsequent evaluation by tracking software was also implemented to increase throughput. The dopaminergic regulation of locomotor velocity was confirmed by bromocriptine, a drug whose mechanisms of action to treat Parkinson’s disease is believed to be through the stimulation of nerves that control movement.
Keywords: planaria; parkinsonism; tyrosine hydroxylase; monoiodotyrosine; bromocriptine; antiparkinsonian agent planaria; parkinsonism; tyrosine hydroxylase; monoiodotyrosine; bromocriptine; antiparkinsonian agent
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Prokai, D.; Nguyen, T.; Kamrowski, K.; Chandra, A.; Talamantes, T.; Baxter, L.R.; Prokai, L. An Exploratory Evaluation of Tyrosine Hydroxylase Inhibition in Planaria as a Model for Parkinsonism. Int. J. Mol. Sci. 2013, 14, 23289-23296.

AMA Style

Prokai D, Nguyen T, Kamrowski K, Chandra A, Talamantes T, Baxter LR, Prokai L. An Exploratory Evaluation of Tyrosine Hydroxylase Inhibition in Planaria as a Model for Parkinsonism. International Journal of Molecular Sciences. 2013; 14(12):23289-23296.

Chicago/Turabian Style

Prokai, David; Nguyen, Thinh; Kamrowski, Kurt; Chandra, Ashwin; Talamantes, Tatjana; Baxter, Lewis R.; Prokai, Laszlo. 2013. "An Exploratory Evaluation of Tyrosine Hydroxylase Inhibition in Planaria as a Model for Parkinsonism." Int. J. Mol. Sci. 14, no. 12: 23289-23296.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert