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Int. J. Mol. Sci. 2013, 14(12), 23289-23296; https://doi.org/10.3390/ijms141223289

An Exploratory Evaluation of Tyrosine Hydroxylase Inhibition in Planaria as a Model for Parkinsonism

1
Department of Psychiatry, College of Medicine, University of Florida, Gainesville, FL 32611, USA
2
Department of Pharmacology and Neuroscience, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
Current Address: College of Engineering, University of Texas at Arlington, Arlington, TX 76203, USA
Current Address: College of Arts and Science, University of North Texas, Denton, TX 76203, USA
§
Current Address: Thermo Fisher Scientific, West Palm Beach, FL 33407, USA
||
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 11 October 2013 / Revised: 18 November 2013 / Accepted: 19 November 2013 / Published: 26 November 2013
(This article belongs to the Special Issue Neuroprotective Strategies 2014)
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Abstract

Planaria are the simplest organisms with bilateral symmetry and a central nervous system (CNS) with cephalization; therefore, they could be useful as model organisms to investigate mechanistic aspects of parkinsonism and to screen potential therapeutic agents. Taking advantage of the organism’s anti-tropism towards light, we measured a significantly reduced locomotor velocity in planaria after exposure to 3-iodo-L-tyrosine, an inhibitor of tyrosine hydroxylase that is an enzyme catalyzing the first and rate-limiting step in the biosynthesis of catecholamines. A simple semi-automatic assay using videotaped experiments and subsequent evaluation by tracking software was also implemented to increase throughput. The dopaminergic regulation of locomotor velocity was confirmed by bromocriptine, a drug whose mechanisms of action to treat Parkinson’s disease is believed to be through the stimulation of nerves that control movement. View Full-Text
Keywords: planaria; parkinsonism; tyrosine hydroxylase; monoiodotyrosine; bromocriptine; antiparkinsonian agent planaria; parkinsonism; tyrosine hydroxylase; monoiodotyrosine; bromocriptine; antiparkinsonian agent
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Prokai, D.; Nguyen, T.; Kamrowski, K.; Chandra, A.; Talamantes, T.; Baxter, L.R.; Prokai, L. An Exploratory Evaluation of Tyrosine Hydroxylase Inhibition in Planaria as a Model for Parkinsonism. Int. J. Mol. Sci. 2013, 14, 23289-23296.

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