Int. J. Mol. Sci. 2013, 14(12), 23244-23256; doi:10.3390/ijms141223244
Article

Screening of Peptide Ligands for Pyrroloquinoline Quinone Glucose Dehydrogenase Using Antagonistic Template-Based Biopanning

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Received: 31 July 2013; in revised form: 31 October 2013 / Accepted: 11 November 2013 / Published: 25 November 2013
(This article belongs to the collection Proteins and Protein-Ligand Interactions)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: We have developed a novel method, antagonistic template-based biopanning, for screening peptide ligands specifically recognizing local tertiary protein structures. We chose water-soluble pyrroloquinoline quinone (PQQ) glucose dehydrogenase (GDH-B) as a model enzyme for this screening. Two GDH-B mutants were constructed as antagonistic templates; these have some point mutations to induce disruption of local tertiary structures within the loop regions that are located at near glucose-binding pocket. Using phage display, we selected 12-mer peptides that specifically bound to wild-type GDH-B but not to the antagonistic templates. Consequently, a peptide ligand showing inhibitory activity against GDH-B was obtained. These results demonstrate that the antagonistic template-based biopanning is useful for screening peptide ligands recognizing the specific local tertiary structure of proteins.
Keywords: peptide ligand; phage display; site-directed; inhibitor
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MDPI and ACS Style

Abe, K.; Yoshida, W.; Terada, K.; Yagi-Ishii, Y.; Ferri, S.; Ikebukuro, K.; Sode, K. Screening of Peptide Ligands for Pyrroloquinoline Quinone Glucose Dehydrogenase Using Antagonistic Template-Based Biopanning. Int. J. Mol. Sci. 2013, 14, 23244-23256.

AMA Style

Abe K, Yoshida W, Terada K, Yagi-Ishii Y, Ferri S, Ikebukuro K, Sode K. Screening of Peptide Ligands for Pyrroloquinoline Quinone Glucose Dehydrogenase Using Antagonistic Template-Based Biopanning. International Journal of Molecular Sciences. 2013; 14(12):23244-23256.

Chicago/Turabian Style

Abe, Koichi; Yoshida, Wataru; Terada, Kotaro; Yagi-Ishii, Yukiko; Ferri, Stefano; Ikebukuro, Kazunori; Sode, Koji. 2013. "Screening of Peptide Ligands for Pyrroloquinoline Quinone Glucose Dehydrogenase Using Antagonistic Template-Based Biopanning." Int. J. Mol. Sci. 14, no. 12: 23244-23256.

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