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Int. J. Mol. Sci. 2013, 14(11), 22982-22996; doi:10.3390/ijms141122982

Differential Proteomic Analysis of Anthers between Cytoplasmic Male Sterile and Maintainer Lines in Capsicum annuum L.

1
College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Zhongkai Road 501, Guangzhou 510225, Guangdong, China
2
College of Horticulture, South China Agricultural University, Wushan Road 483, Guangzhou 510640, Guangdong, China
3
Zunyi Institute of Agricultural Sciences, Zunyi 563102, Guizhou, China
4
Maize Research Institute of Sichuan Agricultural University, Chengdu 611130, Sichuan, China
5
Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Chengdu 611130, Sichuan, China
*
Author to whom correspondence should be addressed.
Received: 24 September 2013 / Revised: 31 October 2013 / Accepted: 5 November 2013 / Published: 20 November 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Cytoplasmic male sterility (CMS), widely used in the production of hybrid seeds, is a maternally inherited trait resulting in a failure to produce functional pollen. In order to identify some specific proteins associated with CMS in pepper, two-dimensional gel electrophoresis (2-DE) was applied to proteomic analysis of anthers/buds between a CMS line (designated NA3) and its maintainer (designated NB3) in Capsicum annuum L. Thirty-three spots showed more than 1.5-fold in either CMS or its maintainer. Based on mass spectrometry, 27 spots representing 23 distinct proteins in these 33 spots were identified. Proteins down-regulated in CMS anthers/buds includes ATP synthase D chain, formate dehydrogenase, alpha-mannosidas, RuBisCO large subunit-binding protein subunit beta, chloroplast manganese stabilizing protein-II, glutathione S-transferase, adenosine kinase isoform 1T-like protein, putative DNA repair protein RAD23-4, putative caffeoyl-CoA 3-O-methyltransferase, glutamine synthetase (GS), annexin Cap32, glutelin, allene oxide cyclase, etc. In CMS anthers/buds, polyphenol oxidase, ATP synthase subunit beta, and actin are up-regulated. It was predicted that male sterility in NA3 might be related to energy metabolism turbulence, excessive ethylene synthesis, and suffocation of starch synthesis. The present study lays a foundation for future investigations of gene functions associated with pollen development and cytoplasmic male sterility, and explores the molecular mechanism of CMS in pepper.
Keywords: Capsicum annuum L.; cytoplasmic male sterility; anther proteome; two-dimensional gel electrophoresis Capsicum annuum L.; cytoplasmic male sterility; anther proteome; two-dimensional gel electrophoresis
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MDPI and ACS Style

Wu, Z.; Cheng, J.; Qin, C.; Hu, Z.; Yin, C.; Hu, K. Differential Proteomic Analysis of Anthers between Cytoplasmic Male Sterile and Maintainer Lines in Capsicum annuum L.. Int. J. Mol. Sci. 2013, 14, 22982-22996.

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