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Int. J. Mol. Sci. 2013, 14(11), 22891-22905; doi:10.3390/ijms141122891

Molecular Cloning and Characterization of a P-Glycoprotein from the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae)

2,*  and 1,*
1 Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China 2 Department of Entomology, University of Kentucky, Lexington, KY 40546-0091, USA
* Authors to whom correspondence should be addressed.
Received: 9 September 2013 / Revised: 1 October 2013 / Accepted: 18 October 2013 / Published: 20 November 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Macrocyclic lactones such as abamectin and ivermectin constitute an important class of broad-spectrum insecticides. Widespread resistance to synthetic insecticides, including abamectin and ivermectin, poses a serious threat to the management of diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), a major pest of cruciferous plants worldwide. P-glycoprotein (Pgp), a member of the ABC transporter superfamily, plays a crucial role in the removal of amphiphilic xenobiotics, suggesting a mechanism for drug resistance in target organisms. In this study, PxPgp1, a putative Pgp gene from P. xylostella, was cloned and characterized. The open reading frame (ORF) of PxPgp1 consists of 3774 nucleotides, which encodes a 1257-amino acid peptide. The deduced PxPgp1 protein possesses structural characteristics of a typical Pgp, and clusters within the insect ABCB1. PxPgp1 was expressed throughout all developmental stages, and showed the highest expression level in adult males. PxPgp1 was highly expressed in midgut, malpighian tubules and testes. Elevated expression of PxPgp1 was observed in P. xylostella strains after they were exposed to the abamectin treatment. In addition, the constitutive expressions of PxPgp1 were significantly higher in laboratory-selected and field-collected resistant strains in comparison to their susceptible counterpart.
Keywords: P-glycoprotein; cloning; characterization; Plutella xylostella; ABC transporter P-glycoprotein; cloning; characterization; Plutella xylostella; ABC transporter
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Tian, L.; Yang, J.; Hou, W.; Xu, B.; Xie, W.; Wang, S.; Zhang, Y.; Zhou, X.; Wu, Q. Molecular Cloning and Characterization of a P-Glycoprotein from the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae). Int. J. Mol. Sci. 2013, 14, 22891-22905.

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