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Int. J. Mol. Sci. 2013, 14(11), 22697-22707; doi:10.3390/ijms141122697
Article

Hyperoside Downregulates the Receptor for Advanced Glycation End Products (RAGE) and Promotes Proliferation in ECV304 Cells via the c-Jun N-Terminal Kinases (JNK) Pathway Following Stimulation by Advanced Glycation End-Products In Vitro

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1 Sericulture & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, NO. 133 Yiheng St. Dongguanzhuang Rd., Tianhe Ditrict, Guangzhou 510610, China 2 Department of Histology and Embryology, Guangzhou Medical University, Guangzhou 510185, China
* Author to whom correspondence should be addressed.
Received: 22 September 2013 / Revised: 27 October 2013 / Accepted: 11 November 2013 / Published: 18 November 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Hyperoside is a major active constituent in many medicinal plants which are traditionally used in Chinese medicines for their neuroprotective, anti-inflammatory and antioxidative effects. The molecular mechanisms underlying these effects are unknown. In this study, quiescent ECV304 cells were treated in vitro with advanced glycation end products (AGEs) in the presence or absence of hyperoside. The results demonstrated that AGEs induced c-Jun N-terminal kinases (JNK) activation and apoptosis in ECV304 cells. Hyperoside inhibited these effects and promoted ECV304 cell proliferation. Furthermore, hyperoside significantly inhibited RAGE expression in AGE-stimulated ECV304 cells, whereas knockdown of RAGE inhibited AGE-induced JNK activation. These results suggested that AGEs may promote JNK activation, leading to viability inhibition of ECV304 cells via the RAGE signaling pathway. These effects could be inhibited by hyperoside. Our findings suggest a novel role for hyperoside in the treatment and prevention of diabetes.
Keywords: hyperoside; RAGE; AGE; JNK; ECV304 hyperoside; RAGE; AGE; JNK; ECV304
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Zhang, Z.; Sethiel, M.S.; Shen, W.; Liao, S.; Zou, Y. Hyperoside Downregulates the Receptor for Advanced Glycation End Products (RAGE) and Promotes Proliferation in ECV304 Cells via the c-Jun N-Terminal Kinases (JNK) Pathway Following Stimulation by Advanced Glycation End-Products In Vitro. Int. J. Mol. Sci. 2013, 14, 22697-22707.

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