The Protective Effect of Bcl-xl Overexpression against Oxidative Stress-Induced Vascular Endothelial Cell Injury and the Role of the Akt/eNOS Pathway
Abstract
:1. Introduction
2. Results and Discussion
2.1. Bcl-xl Overexpression Attenuates Oxidative Injury-Induced Apoptosis in HUVECs
2.2. Protective Effect of Bcl-xl Overexpression on Maintaining the Viability of HUVECs
2.3. Involvement of the Akt/eNOS Pathway in Bcl-xl gene Transfected H2O2-Stimulated HUVECs
2.4. Discussion
3. Experimental Section
3.1. Culturing of HUVECs
3.2. Generation of Recombinant Adenovirus Vectors
3.3. HUVECs Infection with Recombinant Adenovirus
3.4. Treatment of HUVECs with Hydrogen Peroxide
3.5. Analysis of Apoptosis by Hoechst 33,258 and Annexin V/PI Staining
3.6. Analysis of Protein Levels by Western Blotting
3.7. Cell Viability Assay
3.8. Immunocytochemical Detection
3.9. Scratching Assay
3.10. Statistical Analysis
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Ni, L.; Li, T.; Liu, B.; Song, X.; Yang, G.; Wang, L.; Miao, S.; Liu, C. The Protective Effect of Bcl-xl Overexpression against Oxidative Stress-Induced Vascular Endothelial Cell Injury and the Role of the Akt/eNOS Pathway. Int. J. Mol. Sci. 2013, 14, 22149-22162. https://doi.org/10.3390/ijms141122149
Ni L, Li T, Liu B, Song X, Yang G, Wang L, Miao S, Liu C. The Protective Effect of Bcl-xl Overexpression against Oxidative Stress-Induced Vascular Endothelial Cell Injury and the Role of the Akt/eNOS Pathway. International Journal of Molecular Sciences. 2013; 14(11):22149-22162. https://doi.org/10.3390/ijms141122149
Chicago/Turabian StyleNi, Leng, Tianjia Li, Bao Liu, Xitao Song, Genhuan Yang, Linfang Wang, Shiying Miao, and Changwei Liu. 2013. "The Protective Effect of Bcl-xl Overexpression against Oxidative Stress-Induced Vascular Endothelial Cell Injury and the Role of the Akt/eNOS Pathway" International Journal of Molecular Sciences 14, no. 11: 22149-22162. https://doi.org/10.3390/ijms141122149