NF-κB-Targeted Anti-Inflammatory Activity of Prunella vulgaris var. lilacina in Macrophages RAW 264.7
Abstract
:1. Introduction
2. Results and Discussion
2.1. Effect of P. vulgaris var. lilacina on Viability of RAW 264.7 Cells
2.2. Effect of P. vulgaris var. lilacina on LPS-Induced NO and PGE2 Production
2.3. Effect of P. vulgaris var. lilacina on LPS-Induced iNOS and COX-2 Expression
2.4. Effect of P. vulgaris var. lilacina Fractions on TNFα Production
2.5. Effects of P. vulgaris var. lilacina on NF-κB Activity and Translocation of NF-κB Subunits
2.6. Identification of Components
3. Experimental Section
3.1. Reagents
3.2. Sample Preparation and Extraction
3.3. Cell Culture and Viability
3.4. Nitrite Measurement
3.5. Prostaglandin E2 Measurement
3.6. Real-Time Reverse Transcription Polymerase Chain Reaction Analysis
3.7. Immunoblotting
3.8. Cytokine Determinations
3.9. Transfection of RAW 264.7 Cells with pNF-κB Luciferase Vector
3.10. Measurement of NF-κB Luciferase Activity
3.11. Gas Chromatography-Mass Spectrometry Analysis
3.12. Statistical Analysis
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Hwang, Y.-J.; Lee, E.-J.; Kim, H.-R.; Hwang, K.-A. NF-κB-Targeted Anti-Inflammatory Activity of Prunella vulgaris var. lilacina in Macrophages RAW 264.7. Int. J. Mol. Sci. 2013, 14, 21489-21503. https://doi.org/10.3390/ijms141121489
Hwang Y-J, Lee E-J, Kim H-R, Hwang K-A. NF-κB-Targeted Anti-Inflammatory Activity of Prunella vulgaris var. lilacina in Macrophages RAW 264.7. International Journal of Molecular Sciences. 2013; 14(11):21489-21503. https://doi.org/10.3390/ijms141121489
Chicago/Turabian StyleHwang, Yu-Jin, Eun-Ju Lee, Haeng-Ran Kim, and Kyung-A Hwang. 2013. "NF-κB-Targeted Anti-Inflammatory Activity of Prunella vulgaris var. lilacina in Macrophages RAW 264.7" International Journal of Molecular Sciences 14, no. 11: 21489-21503. https://doi.org/10.3390/ijms141121489