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Int. J. Mol. Sci. 2013, 14(10), 20478-20491; doi:10.3390/ijms141020478
Article

Transcriptome Comparative Profiling of Barley eibi1 Mutant Reveals Pleiotropic Effects of HvABCG31 Gene on Cuticle Biogenesis and Stress Responsive Pathways

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Received: 2 September 2013; in revised form: 26 September 2013 / Accepted: 26 September 2013 / Published: 14 October 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Wild barley eibi1 mutant with HvABCG31 gene mutation has low capacity to retain leaf water, a phenotype associated with reduced cutin deposition and a thin cuticle. To better understand how such a mutant plant survives, we performed a genome-wide gene expression analysis. The leaf transcriptomes between the near-isogenic lines eibi1 and the wild type were compared using the 22-k Barley1 Affymetrix microarray. We found that the pleiotropic effect of the single gene HvABCG31 mutation was linked to the co-regulation of metabolic processes and stress-related system. The cuticle development involved cytochrome P450 family members and fatty acid metabolism pathways were significantly up-regulated by the HvABCG31 mutation, which might be anticipated to reduce the levels of cutin monomers or wax and display conspicuous cuticle defects. The candidate genes for responses to stress were induced by eibi1 mutant through activating the jasmonate pathway. The down-regulation of co-expressed enzyme genes responsible for DNA methylation and histone deacetylation also suggested that HvABCG31 mutation may affect the epigenetic regulation for barley development. Comparison of transcriptomic profiling of barley under biotic and abiotic stresses revealed that the functions of HvABCG31 gene to high-water loss rate might be different from other osmotic stresses of gene mutations in barley. The transcriptional profiling of the HvABCG31 mutation provided candidate genes for further investigation of the physiological and developmental changes caused by the mutant.
Keywords: Affymetrix GeneChip; barley; eibi1/HvABCG31 mutant; transcriptome analysis Affymetrix GeneChip; barley; eibi1/HvABCG31 mutant; transcriptome analysis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Yang, Z.; Zhang, T.; Lang, T.; Li, G.; Chen, G.; Nevo, E. Transcriptome Comparative Profiling of Barley eibi1 Mutant Reveals Pleiotropic Effects of HvABCG31 Gene on Cuticle Biogenesis and Stress Responsive Pathways. Int. J. Mol. Sci. 2013, 14, 20478-20491.

AMA Style

Yang Z, Zhang T, Lang T, Li G, Chen G, Nevo E. Transcriptome Comparative Profiling of Barley eibi1 Mutant Reveals Pleiotropic Effects of HvABCG31 Gene on Cuticle Biogenesis and Stress Responsive Pathways. International Journal of Molecular Sciences. 2013; 14(10):20478-20491.

Chicago/Turabian Style

Yang, Zujun; Zhang, Tao; Lang, Tao; Li, Guangrong; Chen, Guoxiong; Nevo, Eviatar. 2013. "Transcriptome Comparative Profiling of Barley eibi1 Mutant Reveals Pleiotropic Effects of HvABCG31 Gene on Cuticle Biogenesis and Stress Responsive Pathways." Int. J. Mol. Sci. 14, no. 10: 20478-20491.



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