Next Article in Journal
Eleven Novel Polymorphic Microsatellite Loci for Oval Squid Sepioteuthis Lessoniana (Shiro-Ika Type)
Previous Article in Journal
Nematicons and Their Electro-Optic Control: Light Localization and Signal Readdressing via Reorientation in Liquid Crystals
Int. J. Mol. Sci. 2013, 14(10), 19951-19970; doi:10.3390/ijms141019951
Article

CD8+ T Cell-Induced Expression of Tissue Inhibitor of Metalloproteinses-1 Exacerbated Osteoarthritis

1
, 2
, 3
, 4
, 5
, 6
, 7
, 2
 and 8,*
1 Department of Nursing, Chung Hwa University of Medical Technology, Tainan 717, Taiwan 2 Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan 3 Department of Microbiology, School of Medicine, China Medical University, Taichung 404, Taiwan 4 Institute of Basic Medical Science, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan 5 Department of Nursing, Chang Gung University of Technology, Puzih, Chiayi County 613, Taiwan 6 Department of Orthopedics, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan 7 Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan 8 Department of Orthopedic Surgery, Tainan Hospital, Department of Health, Executive Yuan, Tainan 700, Taiwan
* Author to whom correspondence should be addressed.
Received: 27 July 2013 / Revised: 11 September 2013 / Accepted: 22 September 2013 / Published: 8 October 2013
(This article belongs to the Section Molecular Pathology)
View Full-Text   |   Download PDF [1181 KB, uploaded 19 June 2014]   |   Browse Figures

Abstract

Despites the fact that T cells are involved in the pathogenesis of osteoarthritis (OA) little is known about the roles of CD8+ T cells in this disease. We investigated the effects of CD8+ T cells and the expression of tissue inhibitor of metalloproteinases 1 (TIMP-1) on joint pathology. Using anterior cruciate ligament-transection (ACLT), OA was induced in mice. The knee joints were histologically assessed for manifestations of OA. The CD8+ T cells from splenocytes and synovium were flow-cytometrically and immunochemically evaluated, respectively. Local expression of TIMP-1, matrix metalloproteinase (MMP)-13, and VEGF were examined. Cartilage degeneration was slower in CD8+ T cell knockout mice than in control mice. CD8+ T cells were activated once OA was initiated and expanded during OA progression. More CD8+ T cells from splenocytes expressed TIMP-1 in ACLT-group mice than in Sham-group mice. The number of TIMP-1-expressing CD8+ T cells in OA mice correlated with the disease severity. TIMP-1 expression in cartilage was co-localized with that of MMP-13 and VEGF. TIMP-1 protein was detected in synovium in which angiogenesis occurred. During the pathogenesis of OA, the expression of TIMP-1, VEGF and MMP-13 accompanying with CD8+ T cells activation were increased. Furthermore, inhibiting the expression of TIMP-1 in joints could retard the progression of OA.
Keywords: CD8+ T cells; osteoarthritis; TIMP-1; VEGF; MMP-13 CD8+ T cells; osteoarthritis; TIMP-1; VEGF; MMP-13
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Export to BibTeX |
EndNote


MDPI and ACS Style

Hsieh, J.-L.; Shiau, A.-L.; Lee, C.-H.; Yang, S.-J.; Lee, B.-O.; Jou, I.-M.; Wu, C.-L.; Chen, S.-H.; Shen, P.-C. CD8+ T Cell-Induced Expression of Tissue Inhibitor of Metalloproteinses-1 Exacerbated Osteoarthritis. Int. J. Mol. Sci. 2013, 14, 19951-19970.

View more citation formats

Supplement

Related Articles

Article Metrics

Comments

Citing Articles

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert