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Eleven Novel Polymorphic Microsatellite Loci for Oval Squid Sepioteuthis Lessoniana (Shiro-Ika Type)
Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8528, Japan
Tokushima Agriculture, Forestry and Fisheries Technology Support Center, Naruto, Tokushima 771-0361, Japan
Gifu Prefectural Research Institute for Freshwater Fish and Aquatic Environments, Gero, Gifu 509-2592, Japan
* Author to whom correspondence should be addressed.
Received: 9 August 2013; in revised form: 5 September 2013 / Accepted: 25 September 2013 / Published: 8 October 2013
Abstract: The oval squid Sepioteuthis lessoniana is one of the most economically important squid species in Japan; however, its population structure is poorly understood due to the lack of hypervariable markers. Such information is critical for managing sustainable fisheries, as well as for ensuring the existence of wild S. lessoniana stocks. Eleven candidate microsatellite loci were isolated from a small insert genomic DNA library. Polymorphisms in these 11 loci were screened in 24 wild individuals. The number of alleles per locus was found to range from 5 to 19 alleles, and the observed heterozygosity ranged from 0.292 to 0.958. No evidence for linkage disequilibrium was detected among all the loci. The genotypic proportions conformed to Hardy-Weinberg equilibrium, except at one locus. In conclusion, these polymorphic microsatellite loci may be used to develop a genetic framework to manage S. lessoniana in the future.
Keywords: microsatellite loci; oval squid; genetic structure; Sepioteuthis lessoniana
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Tomano, S.; Ahmad-Syazni, K.; Ueta, Y.; Ohara, K.; Umino, T. Eleven Novel Polymorphic Microsatellite Loci for Oval Squid Sepioteuthis Lessoniana (Shiro-Ika Type). Int. J. Mol. Sci. 2013, 14, 19971-19975.
Tomano S, Ahmad-Syazni K, Ueta Y, Ohara K, Umino T. Eleven Novel Polymorphic Microsatellite Loci for Oval Squid Sepioteuthis Lessoniana (Shiro-Ika Type). International Journal of Molecular Sciences. 2013; 14(10):19971-19975.
Tomano, Satoshi; Ahmad-Syazni, Kamarudin; Ueta, Yukio; Ohara, Kenichi; Umino, Tetsuya. 2013. "Eleven Novel Polymorphic Microsatellite Loci for Oval Squid Sepioteuthis Lessoniana (Shiro-Ika Type)." Int. J. Mol. Sci. 14, no. 10: 19971-19975.