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Preparation and Characterization of Nanoliposomes Entrapping Medium-Chain Fatty Acids and Vitamin C by Lyophilization
Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China
Ocean Research Center of Zhoushan, Zhejiang University, Zhoushan 316021, China
State Key Laboratory of Food Science and Technology, Department of Life Science and Food Engineering, Nanchang University, Nanchang 330047, China
* Author to whom correspondence should be addressed.
Received: 15 July 2013; in revised form: 12 September 2013 / Accepted: 22 September 2013 / Published: 30 September 2013
Abstract: The complex nanoliposomes encapsulating both a hydrophilic drug vitamin C (vit C) and hydrophobic drug medium-chain fatty acids (MCFAs) was prepared by combining double emulsion method with dynamic high pressure microfluidization. The complex nanoliposomes was further freeze-dried under −86 °C for 48 h with sucrose at the sucrose/lipids ratio of 2:1(w/w) in order to enhance its stability. The freeze-dried complex nanoliposomes under the suitable conditions exhibited high entrapment efficiency of MCFAs (44.26 ± 3.34)%, relatively high entrapment efficiency of vit C (62.25 ± 3.43)%, low average size diameter (110.4 ± 7.28) nm and good storage stability at 4 °C for 60 days with slight changes in mean particle diameter and drug entrapment efficiencies. The results of transmission electron microscopy of freeze-dried complex nanoliposomes also showed that the freeze-dried samples with sucrose were stable without great increase in their particle sizes and without destroying their spherical shape. The results indicated that sucrose presented well protection effects in MCFAs-vit C complex nanoliposomes, suggesting the possibility of further usage in commercial liposomes.
Keywords: medium-chain fatty acids; vitamin C; double emulsion; dynamic high pressure microfluidization; lyophilization
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Yang, S.; Liu, C.; Liu, W.; Yu, H.; Zheng, H.; Zhou, W.; Hu, Y. Preparation and Characterization of Nanoliposomes Entrapping Medium-Chain Fatty Acids and Vitamin C by Lyophilization. Int. J. Mol. Sci. 2013, 14, 19763-19773.
Yang S, Liu C, Liu W, Yu H, Zheng H, Zhou W, Hu Y. Preparation and Characterization of Nanoliposomes Entrapping Medium-Chain Fatty Acids and Vitamin C by Lyophilization. International Journal of Molecular Sciences. 2013; 14(10):19763-19773.
Yang, Shuibing; Liu, Chengmei; Liu, Wei; Yu, Haixia; Zheng, Huijuan; Zhou, Wei; Hu, Yaqin. 2013. "Preparation and Characterization of Nanoliposomes Entrapping Medium-Chain Fatty Acids and Vitamin C by Lyophilization." Int. J. Mol. Sci. 14, no. 10: 19763-19773.