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Pharmacokinetics of Dibutyl Phthalate (DBP) in the Rat Determined by UPLC-MS/MS
Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan
School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan
Department of Education and Research, Taipei City Hospital, Taipei 106, Taiwan
* Author to whom correspondence should be addressed.
Received: 10 September 2012; in revised form: 12 December 2012 / Accepted: 18 December 2012 / Published: 4 January 2013
Abstract: Dibutyl phthalate (DBP) is commonly used to increase the ﬂexibility of plastics in industrial products. However, several plasticizers have been illegally used as clouding agents to increase dispersion of aqueous matrix in beverages. This study thus develops a rapid and validated analytical method by ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) for the evaluation of pharmacokinetics of DBP in free moving rats. The UPLC-MS/MS system equipped with positive electrospray ionization (ESI) source in multiple reaction monitoring (MRM) mode was used to monitor m/z 279.25→148.93 transitions for DBP. The limit of quantiﬁcation for DBP in rat plasma and feces was 0.05 µg/mL and 0.125 µg/g, respectively. The pharmacokinetic results demonstrate that DBP appeared to have a two-compartment model in the rats; the area under concentration versus time (AUC) was 57.8 ± 5.93 min μg/mL and the distribution and elimination half-life (t1/2,α and t1/2,β) were 5.77 ± 1.14 and 217 ± 131 min, respectively, after DBP administration (30 mg/kg, i.v.). About 0.18% of the administered dose was recovered from the feces within 48 h. The pharmacokinetic behavior demonstrated that DBP was quickly degraded within 2 h, suggesting a rapid metabolism low fecal cumulative excretion in the rat.
Keywords: dibutyl phthalate (DBP); liquid chromatography with tandem mass spectrometry (LC-MS/MS); pharmacokinetics; plasticizer
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MDPI and ACS Style
Chang, L.-W.; Hou, M.-L.; Tsai, T.-H. Pharmacokinetics of Dibutyl Phthalate (DBP) in the Rat Determined by UPLC-MS/MS. Int. J. Mol. Sci. 2013, 14, 836-849.
Chang L-W, Hou M-L, Tsai T-H. Pharmacokinetics of Dibutyl Phthalate (DBP) in the Rat Determined by UPLC-MS/MS. International Journal of Molecular Sciences. 2013; 14(1):836-849.
Chang, Li-Wen; Hou, Mei-Ling; Tsai, Tung-Hu. 2013. "Pharmacokinetics of Dibutyl Phthalate (DBP) in the Rat Determined by UPLC-MS/MS." Int. J. Mol. Sci. 14, no. 1: 836-849.