Nuclear Factor-κB (NF-κB) Regulates the Expression of Human Testis-Enriched Leucine-Rich Repeats and WD Repeat Domain Containing 1 (LRWD1) Gene
Abstract
:1. Introduction
2. Results and Discussion
2.1. Promoter Activity in the 5′-Flanking Region of the Testis-Enriched LRWD1 Gene
2.2. The LRWD1 Promoter Region Includes an Evolutionarily Conserved κB Site
2.3. The κB Site within the LRWD1 Promoter Binds NF-κB in Testicular Germ Cell Nuclei
2.4. NF-κB Binds to the LRWD1 Promoter and Regulates Its Activity
2.5. Activation of NF-κB with LPS Increases LRWD1 Expression
2.6. NF-κB Stimulates LRWD1 Promoter Activity
3. Experimental Section
3.1. LRWD1 Expression Assay by RT-PCR
3.2. Cell Culture and Isolation of Genomic DNA
3.3. Sources of Plasmids and Plasmid Construction
3.4. Transient Transfection and Luciferase Reporter Gene Assay
3.5. Western Blot Analysis
3.6. Chromatin Immunoprecipitation (ChIP)
3.7. Gel Electrophoretic Mobility Shift Assay (EMSA)
3.8. Statistical Analysis and Bioinformatics Software
4. Discussion and Conclusions
Acknowledgments
- Conflict of InterestThe authors declare that they have no conflict of interest.
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Teng, Y.-N.; Chuang, P.-J.; Liu, Y.-W. Nuclear Factor-κB (NF-κB) Regulates the Expression of Human Testis-Enriched Leucine-Rich Repeats and WD Repeat Domain Containing 1 (LRWD1) Gene. Int. J. Mol. Sci. 2013, 14, 625-639. https://doi.org/10.3390/ijms14010625
Teng Y-N, Chuang P-J, Liu Y-W. Nuclear Factor-κB (NF-κB) Regulates the Expression of Human Testis-Enriched Leucine-Rich Repeats and WD Repeat Domain Containing 1 (LRWD1) Gene. International Journal of Molecular Sciences. 2013; 14(1):625-639. https://doi.org/10.3390/ijms14010625
Chicago/Turabian StyleTeng, Yen-Ni, Po-Jung Chuang, and Yo-Wen Liu. 2013. "Nuclear Factor-κB (NF-κB) Regulates the Expression of Human Testis-Enriched Leucine-Rich Repeats and WD Repeat Domain Containing 1 (LRWD1) Gene" International Journal of Molecular Sciences 14, no. 1: 625-639. https://doi.org/10.3390/ijms14010625