Int. J. Mol. Sci. 2013, 14(1), 1999-2010; doi:10.3390/ijms14011999
Article

Quantitative and Chemical Fingerprint Analysis for the Quality Evaluation of Receptaculum Nelumbinis by RP-HPLC Coupled with Hierarchical Clustering Analysis

1 Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China 2 Department of Science, Fujian Institute of Education, Fuzhou 350001, China 3 Fujian Academy of Agricultural Sciences, Fuzhou 350013, China
* Author to whom correspondence should be addressed.
Received: 28 September 2012; in revised form: 10 January 2013 / Accepted: 11 January 2013 / Published: 21 January 2013
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Abstract: A simple and reliable method of high-performance liquid chromatography with photodiode array detection (HPLC-DAD) was developed to evaluate the quality of Receptaculum Nelumbinis (dried receptacle of Nelumbo nucifera) through establishing chromatographic fingerprint and simultaneous determination of five flavonol glycosides, including hyperoside, isoquercitrin, quercetin-3-O-β-d-glucuronide, isorhamnetin-3-O-β-d-galactoside and syringetin-3-O-β-d-glucoside. In quantitative analysis, the five components showed good regression (R > 0.9998) within linear ranges, and their recoveries were in the range of 98.31%–100.32%. In the chromatographic fingerprint, twelve peaks were selected as the characteristic peaks to assess the similarities of different samples collected from different origins in China according to the State Food and Drug Administration (SFDA) requirements. Furthermore, hierarchical cluster analysis (HCA) was also applied to evaluate the variation of chemical components among different sources of Receptaculum Nelumbinis in China. This study indicated that the combination of quantitative and chromatographic fingerprint analysis can be readily utilized as a quality control method for Receptaculum Nelumbinis and its related traditional Chinese medicinal preparations.
Keywords: Receptaculum Nelumbinis; flavonoids compounds; fingerprint analysis; chemometric analysis; quality control

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MDPI and ACS Style

Wu, Y.-B.; Zheng, L.-J.; Yi, J.; Wu, J.-G.; Chen, T.-Q.; Wu, J.-Z. Quantitative and Chemical Fingerprint Analysis for the Quality Evaluation of Receptaculum Nelumbinis by RP-HPLC Coupled with Hierarchical Clustering Analysis. Int. J. Mol. Sci. 2013, 14, 1999-2010.

AMA Style

Wu Y-B, Zheng L-J, Yi J, Wu J-G, Chen T-Q, Wu J-Z. Quantitative and Chemical Fingerprint Analysis for the Quality Evaluation of Receptaculum Nelumbinis by RP-HPLC Coupled with Hierarchical Clustering Analysis. International Journal of Molecular Sciences. 2013; 14(1):1999-2010.

Chicago/Turabian Style

Wu, Yan-Bin; Zheng, Li-Jun; Yi, Jun; Wu, Jian-Guo; Chen, Ti-Qiang; Wu, Jin-Zhong. 2013. "Quantitative and Chemical Fingerprint Analysis for the Quality Evaluation of Receptaculum Nelumbinis by RP-HPLC Coupled with Hierarchical Clustering Analysis." Int. J. Mol. Sci. 14, no. 1: 1999-2010.

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