Int. J. Mol. Sci. 2012, 13(9), 12082-12093; doi:10.3390/ijms130912082
Article

Development of New Microsatellite Markers for Salvia officinalis L. and Its Potential Use in Conservation-Genetic Studies of Narrow Endemic Salvia brachyodon Vandas

1email, 2email, 3email, 3email, 1email, 4 and 1,* email
Received: 9 August 2012; in revised form: 30 August 2012 / Accepted: 5 September 2012 / Published: 24 September 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Nine new microsatellite markers (SSR) were isolated from Salvia officinalis L. A total of 125 alleles, with 8 to 21 alleles per locus, were detected in a natural population from the east Adriatic coast. The observed heterozygosity, expected heterozygosity, and polymorphic information content ranged from 0.46 to 0.83, 0.73 to 0.93 and 0.70 to 0.92, respectively. New microsatellite markers, as well as previously published markers, were tested for cross-amplification in Salvia brachyodon Vandas, a narrow endemic species known to be present in only two localities on the Balkan Peninsula. Out of 30 microsatellite markers tested on the natural S. brachyodon population, 15 were successfully amplified. To obtain evidence of recent bottleneck events in the populations of both species, observed genetic diversity (HE) was compared to the expected genetic diversity at mutation-drift equilibrium (HEQ) and calculated from the observed number of alleles using a two-phased mutation model (TPM). Recent bottleneck events were detected only in the S. brachyodon population. This result suggests the need to reconsider the current threat category of this endemic species.
Keywords: common sage; conservation; cross-amplification; Dalmatian sage; genetic bottleneck; microsatellites; Lamiaceae; population genetics; short-tooth sage
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MDPI and ACS Style

Radosavljević, I.; Satovic, Z.; Jakse, J.; Javornik, B.; Greguraš, D.; Jug-Dujaković, M.; Liber, Z. Development of New Microsatellite Markers for Salvia officinalis L. and Its Potential Use in Conservation-Genetic Studies of Narrow Endemic Salvia brachyodon Vandas. Int. J. Mol. Sci. 2012, 13, 12082-12093.

AMA Style

Radosavljević I, Satovic Z, Jakse J, Javornik B, Greguraš D, Jug-Dujaković M, Liber Z. Development of New Microsatellite Markers for Salvia officinalis L. and Its Potential Use in Conservation-Genetic Studies of Narrow Endemic Salvia brachyodon Vandas. International Journal of Molecular Sciences. 2012; 13(9):12082-12093.

Chicago/Turabian Style

Radosavljević, Ivan; Satovic, Zlatko; Jakse, Jernej; Javornik, Branka; Greguraš, Danijela; Jug-Dujaković, Marija; Liber, Zlatko. 2012. "Development of New Microsatellite Markers for Salvia officinalis L. and Its Potential Use in Conservation-Genetic Studies of Narrow Endemic Salvia brachyodon Vandas." Int. J. Mol. Sci. 13, no. 9: 12082-12093.


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