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• Radosavljević, I
• Satovic, Z
• Jakse, J
• Javornik, B
• Greguraš, D
• Jug-Dujaković, M
• Liber, Z
• [+] on Google Scholar
• Radosavljević, I
• Satovic, Z
• Jakse, J
• Javornik, B
• Greguraš, D
• Jug-Dujaković, M
• Liber, Z
• [+] on PubMed
• Radosavljević, I
• Satovic, Z
• Jakse, J
• Javornik, B
• Greguraš, D
• Jug-Dujaković, M
• Liber, Z

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Int. J. Mol. Sci. 2012, 13(9), 12082-12093; doi:10.3390/ijms130912082

Article

Development of New Microsatellite Markers for Salvia officinalis L. and Its Potential Use in Conservation-Genetic Studies of Narrow Endemic Salvia brachyodon Vandas

Ivan Radosavljević ¹ , Zlatko Satovic ² , Jernej Jakse ³ , Branka Javornik ³ , Danijela Greguraš ¹ , Marija Jug-Dujaković ⁴  and Zlatko Liber ^1,*

¹ Department of Botany, Division of Biology, Faculty of Science, University of Zagreb, Marulićev trg 9A, 10000 Zagreb, Croatia ² Department of Seed Science and Technology, Faculty of Agriculture, University of Zagreb, Svetošimunska 25, 10000 Zagreb, Croatia ³ Centre for Plant Biotechnology and Breeding, Agronomy Department, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia ⁴ Institute for Adriatic Crops and Karst Reclamation, Put Duilova 11, 21000 Split, Croatia

* Author to whom correspondence should be addressed.

Received: 9 August 2012; in revised form: 30 August 2012 / Accepted: 5 September 2012 / Published: 24 September 2012

(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)

Download PDF Full-Text [780 KB, uploaded 24 September 2012 11:40 CEST]

Abstract: Nine new microsatellite markers (SSR) were isolated from Salvia officinalis L. A total of 125 alleles, with 8 to 21 alleles per locus, were detected in a natural population from the east Adriatic coast. The observed heterozygosity, expected heterozygosity, and polymorphic information content ranged from 0.46 to 0.83, 0.73 to 0.93 and 0.70 to 0.92, respectively. New microsatellite markers, as well as previously published markers, were tested for cross-amplification in Salvia brachyodon Vandas, a narrow endemic species known to be present in only two localities on the Balkan Peninsula. Out of 30 microsatellite markers tested on the natural S. brachyodon population, 15 were successfully amplified. To obtain evidence of recent bottleneck events in the populations of both species, observed genetic diversity (H_E) was compared to the expected genetic diversity at mutation-drift equilibrium (H_EQ) and calculated from the observed number of alleles using a two-phased mutation model (TPM). Recent bottleneck events were detected only in the S. brachyodon population. This result suggests the need to reconsider the current threat category of this endemic species.

Keywords: common sage; conservation; cross-amplification; Dalmatian sage; genetic bottleneck; microsatellites; Lamiaceae; population genetics; short-tooth sage

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