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Int. J. Mol. Sci. 2012, 13(9), 11497-11519; doi:10.3390/ijms130911497
Article

Characterization of Erysiphe necator-Responsive Genes in Chinese Wild Vitis quinquangularis

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Received: 21 June 2012 / Revised: 26 August 2012 / Accepted: 30 August 2012 / Published: 13 September 2012
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Abstract

Powdery mildew (PM), caused by fungus Erysiphe necator, is one of the most devastating diseases of grapevine. To better understand grapevine-PM interaction and provide candidate resources for grapevine breeding, a suppression subtractive hybridization (SSH) cDNA library was constructed from E. necator-infected leaves of a resistant Chinese wild Vitis quinquangularis clone “Shang-24”. A total of 492 high quality expressed sequence tags (ESTs) were obtained and assembled into 266 unigenes. Gene ontology (GO) analysis indicated that 188 unigenes could be assigned with at least one GO term in the biological process category, and 176 in the molecular function category. Sequence analysis showed that a large number of these genes were homologous to those involved in defense responses. Genes involved in metabolism, photosynthesis, transport and signal transduction were also enriched in the library. Expression analysis of 13 selected genes by qRT-PCR revealed that most were induced more quickly and intensely in the resistant material “Shang-24” than in the sensitive V. pseudoreticulata clone “Hunan-1” by E. necator infection. The ESTs reported here provide new clues to understand the disease-resistance mechanism in Chinese wild grapevine species and may enable us to investigate E. necator-responsive genes involved in PM resistance in grapevine germplasm.
Keywords: Chinese wild Vitis quinquangularis; Erysiphe necator; SSH; EST; qRT-PCR Chinese wild Vitis quinquangularis; Erysiphe necator; SSH; EST; qRT-PCR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Gao, M.; Niu, J.; Zhao, S.; Jiao, C.; Xu, W.; Fei, Z.; Wang, X. Characterization of Erysiphe necator-Responsive Genes in Chinese Wild Vitis quinquangularis. Int. J. Mol. Sci. 2012, 13, 11497-11519.

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