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Medium Optimization for Exopolysaccharide Production in Liquid Culture of Endophytic Fungus Berkleasmium sp. Dzf12
Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China
Department of Forestry Pathology, College of Forestry, Northwest A & F University, Yangling 712100, China
* Author to whom correspondence should be addressed.
Received: 1 August 2012; in revised form: 24 August 2012 / Accepted: 27 August 2012 / Published: 12 September 2012
Abstract: Berkleasmium sp. Dzf12, an endophytic fungus from Dioscorea zingiberensis, is a high producer of spirobisnaphthalenes with various bioactivities. The exopolysaccharide (EPS) produced by this fungus also shows excellent antioxidant activity. In this study, the experimental designs based on statistics were employed to evaluate and optimize the medium for EPS production in liquid culture of Berkleasmium sp. Dzf12. For increasing EPS yield, the concentrations of glucose, peptone, KH2PO4, MgSO4∙7H2O and FeSO4∙7H2O in medium were optimized using response surface methodology (RSM). Both the fractional factorial design (FFD) and central composite design (CCD) were applied to optimize the main factors which significantly affected EPS production. The concentrations of glucose, peptone and MgSO4∙7H2O were found to be the main effective factors for EPS production by FFD experimental analysis. Based on the further CCD optimization and RSM analysis, a quadratic polynomial regression equation was derived from the EPS yield and three variables. Statistical analysis showed the polynomial regression model was in good agreement with the experimental results with the determination coefficient (adj-R2) as 0.9434. By solving the quadratic regression equation, the optimal concentrations of glucose, peptone and MgSO4∙7H2O for EPS production were determined as 63.80, 20.76 and 2.74 g/L, respectively. Under the optimum conditions, the predicted EPS yield reached the maximum (13.22 g/L). Verification experiment confirmed the validity with the actual EPS yield as 13.97 g/L, which was 6.29-fold in comparison with that (2.22 g/L) in the original basal medium. The results provide the support data for EPS production in large scale and also speed up the application of Berkleasmium sp. Dzf12.
Keywords: medium optimization; endophytic fungus Berkleasmium sp. Dzf12; exopolysaccharide; fractional factorial design; center composite design; response surface methodology
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Li, P.; Xu, L.; Mou, Y.; Shan, T.; Mao, Z.; Lu, S.; Peng, Y.; Zhou, L. Medium Optimization for Exopolysaccharide Production in Liquid Culture of Endophytic Fungus Berkleasmium sp. Dzf12. Int. J. Mol. Sci. 2012, 13, 11411-11426.
Li P, Xu L, Mou Y, Shan T, Mao Z, Lu S, Peng Y, Zhou L. Medium Optimization for Exopolysaccharide Production in Liquid Culture of Endophytic Fungus Berkleasmium sp. Dzf12. International Journal of Molecular Sciences. 2012; 13(9):11411-11426.
Li, Peiqin; Xu, Liang; Mou, Yan; Shan, Tijiang; Mao, Ziling; Lu, Shiqiong; Peng, Youliang; Zhou, Ligang. 2012. "Medium Optimization for Exopolysaccharide Production in Liquid Culture of Endophytic Fungus Berkleasmium sp. Dzf12." Int. J. Mol. Sci. 13, no. 9: 11411-11426.